MicroRNA-217 attenuates intima-media complex thickness of ascending aorta measured by ultrasound bio-microscopy and inhibits inflammation and lipid metabolism in atherosclerotic models of ApoE-/- mice.

Abstract

BACKGROUND:

Little investigation was done to test the efficiency of microRNA-217 (miR-217) on atherosclerosis in vivo.

METHODS:

ApoE-/- mice were used to construct atherosclerotic models and ultrasound bio-microscopy (UBM) was applied to detect the intima-media thickness (IMT) of the ascending aorta. The serum level of miR-217 and correlation with IMT was investigated. After miR-217 mimic administration, the IMT, inflammation, and lipid-associated molecules were assayed.

RESULTS:

The serum level of miR-217 was reduced in ApoE-/- mice and showed a negative correlation with the IMT of the ascending aorta (r2 = 0.5899, p < 0.0001). miR-217 mimic administration attenuated IMT and down-regulated the level of serum triglyceride (TG), total cholesterol (TC), and low-density-lipoprotein cholesterol (LDL-C), while it could up-regulate high-density lipoprotein cholesterol (HDL-C). Inflammation relevant genes, such as F4/80, tumor necrosis factor (TNF)-α, interleukin (IL)-1, IL-6, and monocyte chemoattractant protein (MCP)-1, and lipid metabolism associated gene, such as LDL receptor, class A scavenger receptors (SR-A), scavenger receptor class B type I (SR-BI), CD36, ATP binding cassette subfamily A member 1 (ABCA1), and ATP binding cassette subfamily G member 1 (ABCG1) in the aorta were significantly down-regulated in miR-217 group when compared with atherosclerosis group.

CONCLUSION:

miR-217 could down-regulate IMT and modulate the inflammation and lipid metabolism process, which indicates that miR-217 could be a potential treatment option.

• Authors: Haina Liu, Xia Li, Yanpeng Song, Zhibin Wang
• Journal: Lipids in health and disease (Lipids Health Dis) Vol. 17 Issue 1 Pg. 170 (Jul 24 2018) ISSN: 1476-511X [Electronic] England
• PMID: 30041636 (Publication Type: Journal Article)

Chemical References

• Lipids
• MIRN-217 microRNA, mouse
• MicroRNAs

Topics

• Animals
• Aorta (pathology, physiology)
• Atherosclerosis (genetics, physiopathology)
• Carotid Intima-Media Thickness
• Disease Models, Animal
• Gene Expression Regulation
• Inflammation (genetics)
• Lipid Metabolism (genetics)
• Lipids (blood, genetics)
• Mice, Knockout, ApoE
• MicroRNAs (genetics)
• Microscopy, Acoustic (methods)