Anti-proliferative and anti-invasive effects of garcinol from Garcinia indica on gallbladder carcinoma cells.

Abstract

Garcinol, a natural histone acetyltransferase inhibitor, has been reported to exhibit significant anti-proliferative activity in various cancer cell types. However, no information is available about the anti-cancer effects of garcinol on gallbladder carcinoma cells (GBC). In this study, GBC cells (GBC-SD and NOZ) were treated by garcinol and subjected to Cell Counting Kit-8 (CCK-8), and GBC-SD cells were selected for further transwell chamber assay, quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analysis. Our results indicated that garcinol could significantly inhibit the growth of GBC cells in a dose- and time-dependent manner. It also inhibited the invasion of GBC-SD cells in a dose-dependent manner. Garcinol treatment decreased the activity of matrix metalloproteinase 2 (MMP2) and MMP9 by the downregulation of mRNA levels, and these two enzymes are critical to tumor invasion. Treatment with garcinol also decreased Stat3 and Akt activation in GBC-SD cells. Taken together, the effects of garcinol on GBC-SD cells may be associated with the suppression of Stat3 and Akt signaling pathways, which may contribute to inhibiting their downstream targets such as mRNA levels of MMP2 and MMP9.

• Authors: Yi-Tao Duan, Xiao-Ang Yang, Lian-Ying Fang, Jin-Han Wang, Qiang Liu
• Journal: Die Pharmazie (Pharmazie) Vol. 73 Issue 7 Pg. 413-417 (07 01 2018) ISSN: 0031-7144 [Print] Germany
• PMID: 30001777 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• RNA, Messenger
• STAT3 Transcription Factor
• STAT3 protein, human
• Terpenes
• Proto-Oncogene Proteins c-akt
• MMP2 protein, human
• Matrix Metalloproteinase 2
• MMP9 protein, human
• Matrix Metalloproteinase 9
• garcinol

Topics

• Antineoplastic Agents, Phytogenic (administration & dosage, isolation & purification, pharmacology)
• Cell Line, Tumor
• Cell Proliferation
• Dose-Response Relationship, Drug
• Gallbladder Neoplasms (drug therapy, pathology)
• Garcinia (chemistry)
• Humans
• Matrix Metalloproteinase 2 (genetics)
• Matrix Metalloproteinase 9 (genetics)
• Neoplasm Invasiveness (prevention & control)
• Proto-Oncogene Proteins c-akt (metabolism)
• RNA, Messenger (metabolism)
• Real-Time Polymerase Chain Reaction
• STAT3 Transcription Factor (metabolism)
• Terpenes (administration & dosage, isolation & purification, pharmacology)
• Time Factors