Anti-Inflammatory Effects of Taraxasterol on LPS-Stimulated Human Umbilical Vein Endothelial Cells.

Abstract

In the present study, we used the human umbilical vein endothelial cells (HUVECs) to investigate the anti-inflammatory effects and mechanism of taraxasterol on vascular inflammation. HUVECs were pre-treated with taraxasterol 1 h before lipopolysaccharide (LPS) treatment. The concentrations of TNF-α, IL-8, PGE2, and NO were measured. The expression of VCAM-1, ICAM-1, iNOS, COX-2, NF-κB, and LXRα was detected by western blot analysis. The results showed that taraxasterol not only reduced the production of TNF-α, IL-8, PGE2, and NO induced by LPS, but also reduced the expression of iNOS and COX-2. Taraxasterol also suppressed LPS-induced NF-κB activation and VCAM-1 and ICAM-1 expression. Furthermore, taraxasterol concentration-dependently increased the expression of LXRα. The inhibition of taraxasterol on TNF-α, IL-8, PGE2, and NO production can be reversed by geranylgeranyl diphosphate (GGPP, the LXRα inhibitor). Here, we found that taraxasterol inhibited vascular inflammation through activating LXRα.

• Authors: Feng Zheng, Xiaomeng Dong, Xiangzhu Meng
• Journal: Inflammation (Inflammation) Vol. 41 Issue 5 Pg. 1755-1761 (Oct 2018) ISSN: 1573-2576 [Electronic] United States
• PMID: 29951871 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• Lipopolysaccharides
• Liver X Receptors
• NF-kappa B
• Sterols
• Triterpenes
• taraxasterol

Topics

• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Cells, Cultured
• Cytokines (drug effects)
• Human Umbilical Vein Endothelial Cells (drug effects)
• Humans
• Inflammation (drug therapy)
• Lipopolysaccharides
• Liver X Receptors
• NF-kappa B (metabolism)
• Sterols (pharmacology, therapeutic use)
• Triterpenes (pharmacology, therapeutic use)