The molecular mechanisms behind aneurysmal subarachnoid haemorrhage (aSAH) are still poorly understood. Expression patterns of
miRNAs may help elucidate the post-transcriptional gene expression in aSAH. Here, we evaluate the global
miRNAs expression profile (miRnome) of patients with aSAH to identify potential
biomarkers. We collected 33 peripheral blood samples (27 patients with
cerebral aneurysm, collected 7 to 10 days after the haemorrhage, when usually is the
cerebral vasospasm risk peak, and six controls). Then, were performed small
RNA sequencing using an Illumina Next Generation Sequencing (NGS) platform. Differential expression analysis identified eight differentially expressed
miRNAs. Among them, three were identified being up-regulated, and five down-regulated. miR-486-5p was the most abundant expressed and is associated with poor neurological admission status. In silico
miRNA gene target prediction showed 148 genes associated with at least two differentially expressed
miRNAs. Among these, THBS1 and VEGFA, known to be related to
thrombospondin and
vascular endothelial growth factor. Moreover, MYC gene was found to be regulated by four
miRNAs, suggesting an important role in aneurysmal subarachnoid haemorrhage. Additionally, 15 novel
miRNAs were predicted being expressed only in aSAH, suggesting possible involvement in
aneurysm pathogenesis. These findings may help the identification of novel
biomarkers of clinical interest.