Abstract | BACKGROUND: RESULTS: Three engineered strains were obtained by overexpressing the SAM synthetase gene AcsamS and the cystathionine-γ- lyase gene mecB, and disrupting a SAM dependent methyltransferase gene Acppm1, respectively. Overexpression of AcsamS resulted in fourfold increase of CPC production which reached to 129.7 µg/mL. Disruption of Acppm1 also increased CPC production (up to 135.5 µg/mL) through enhancing the accumulation of intracellular SAM. Finally, an optimum recombinant strain (Acppm1DM-mecBOE) was constructed through overexpressing mecB in the Acppm1 disruption mutant. In this strain, CPC production reached to the maximum value (142.7 µg/mL) which was 5.5-fold of the wild-type level and its improvement was totally independent of methionine stimulation. CONCLUSIONS: In this study, we constructed a recombinant strain in which the improvement of CPC production was totally independent of methionine stimulation. This work provides an economic route for improving CPC production in A. chrysogenum through metabolic engineering.
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Authors | Jiajia Liu, Wenyan Gao, Yuanyuan Pan, Gang Liu |
Journal | Microbial cell factories
(Microb Cell Fact)
Vol. 17
Issue 1
Pg. 87
(Jun 07 2018)
ISSN: 1475-2859 [Electronic] England |
PMID | 29879990
(Publication Type: Journal Article)
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Chemical References |
- Cephalosporins
- cephalosporin C
- Methionine
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Topics |
- Acremonium
(pathogenicity)
- Cephalosporins
(metabolism)
- Metabolic Engineering
(methods)
- Methionine
(metabolism)
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