Fluid
resuscitation could hardly be performed immediately after fatal
hemorrhagic shock in outpatients. We investigated whether
electroacupuncture (EA) at Zusanli (ST36) could prevent fatal
hemorrhagic shock induced
heart failure with delayed fluid
resuscitation and whether the protective role of EA is related to the autonomic nervous system. Sixty Sprague Dawley rats were randomly divided into five groups (n = 12 each): group of
sham hemorrhagic shock (
SHAM), group of EA, group of
sham EA (SEA), group of delayed fluid
resuscitation with EA (EA + DR), and group of delayed fluid
resuscitation with SEA (SEA + DR). After blood loss for 6 hours,
caspase-3 activity and positive rate of TUNEL in EA + DR group were significantly lower than in other
hemorrhagic shock groups (e.g., versus SEA + DR: 0.156 ± 0.039 versus 0.301 ± 0.042; P < 0.05). Immediately EA treatment after the blood loss enhanced the protective effect of delayed
resuscitation on the cardiac tissue of
hemorrhagic shock rats. Considering the significant changes of
epinephrine (137.8 ± 6.9 ng/L versus 98.6 ± 7.4 ng/L; P < 0.05) and
acetylcholine (405 ± 8.6 pmol/L versus 341 ± 10.1 pmol/L; P < 0.05) after EA treatment (SEA + DR versus EA + DR), this cardiac protective effect may be related to regulation of the autonomic nervous system.