Abstract | PURPOSE:
Cell penetrating peptides (CPPs) were widely used as motifs for drug delivery to tumor. In former study, an RGD reverse sequence dGR was used to develop active-targeting liposome R8dGR-Lip, which showed well penetrating ability and treatment efficiency on glioma model. However, recurrence after tumor resection caused by post-operative residual cancer cells was a huge obstacle in tumor treatment. In consideration of the effective anti- cancer effect of PTX-R8dGR-Lip when treating glioma in former study, we decide to evaluate its pharmacodynamics on tumor resection models, which were more invasive and resistant. METHOD: In vitro, the effectiveness of PTX-R8dGR-Lip in reducing tumor initiating cell ( TIC) was investigated using mammosphere formation. In vivo, the inhibition efficiency of PTX-R8dGR-Lip on C6 glioma recurrence and 4 T1 breast cancer recurrence model were evaluated, including tumor bioluminescence imaging, survival rate and immumohistochemical staining, etc.. RESULTS: C6 mammosphere formation rate of PTX-R8dGR-Lip group was 48.06 ± 2.72%, and 4 T1 mammosphere formation rate of PTX-R8dGR-Lip group was 39.51 ± 4.02% when PBS group was set as 100%. C6 and 4 T1 bioluminescent tumor resected model were established, then effectiveness of different PTX-loaded preparations were evaluated on these two models. PTX-R8dGR-Lip could obviously inhibit tumor recurrence, prolong survival rate and reduce tumor tissue invasion. CONCLUSION: PTX-R8dGR-Lip could reduce post-operative recurrence rate, prolong survival time, and decrease the proliferation of residual cancer cells through regulating the expression of recurrence-related cytokines.
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Authors | Yue Qiu, Qianwen Yu, Yayuan Liu, Jiajing Tang, Xuhui Wang, Zhengze Lu, Zhuping Xu, Qin He |
Journal | Pharmaceutical research
(Pharm Res)
Vol. 35
Issue 7
Pg. 130
(Apr 26 2018)
ISSN: 1573-904X [Electronic] United States |
PMID | 29700620
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- Liposomes
- Oligopeptides
- Peptide Fragments
- Polyethylene Glycols
- arginyl-glycyl-aspartic acid
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Topics |
- Animals
- Antineoplastic Agents
(administration & dosage, metabolism)
- Brain Neoplasms
(drug therapy, metabolism, surgery)
- Breast Neoplasms
(drug therapy, metabolism, surgery)
- Cell Line, Tumor
- Drug Delivery Systems
(methods)
- Female
- Glioma
(drug therapy, metabolism, surgery)
- Humans
- Liposomes
- Mice
- Mice, Inbred BALB C
- Neoplasm Recurrence, Local
(drug therapy, metabolism)
- Oligopeptides
(administration & dosage, metabolism)
- Peptide Fragments
(administration & dosage, metabolism)
- Polyethylene Glycols
(administration & dosage, metabolism)
- Postoperative Care
(methods)
- Rats
- Xenograft Model Antitumor Assays
(methods)
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