The knockdown of the mediator complex subunit MED30 suppresses the proliferation and migration of renal cell carcinoma cells.

Abstract

BACKGROUND:

The mediator complex consists of 33 subunits and plays a central role in transcription. Studies have already described the involvement of individual subunits, especially in carcinogenesis. With regard to the subunit MED30, this has, so far, only been confirmed in gastric and breast carcinoma. The role of MED30 in urological tumours is unknown.

MATERIALS AND METHODS:

First, a database analysis using cBioPortal was performed for the mRNA expression and survival analysis of MED30 in clear cell renal cell carcinoma (ccRCC) and papillary RCC (pRCC). The immunohistochemical analysis (IHC) against MED30 was performed on tissue microarrays (TMA), with benign, ccRCC, pRCC samples, and ccRCC-metastases. Intensity evaluation was performed using the IRS (Immunoreactive Score). The ccRCC cell lines ACHN and A-498 were used for the functional investigation of proliferation, migration, and invasion after the knockdown of MED30 by siRNA.

RESULTS:

In a database analysis by cBioPortal, it was shown that mRNA overexpression of MED30 in the pRCC was significantly associated with a poorer overall survival and progression-free survival. In the IHC, pRCC showed the highest level of MED30 expression, unfortunately without significant results in the survival analysis. The knockdown of MED30 resulted in a significant decrease in proliferation, migration, and invasion in ccRCC.

CONCLUSION:

In summary, MED30 seems to be involved in the progression of the RCC.

• Authors: Isabella Syring, Richard Weiten, Tim Müller, Doris Schmidt, Susanne Steiner, Stefan C Müller, Glen Kristiansen, Jörg Ellinger
• Journal: Annals of diagnostic pathology (Ann Diagn Pathol) Vol. 34 Pg. 18-26 (Jun 2018) ISSN: 1532-8198 [Electronic] United States
• PMID: 29661722 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 Elsevier Inc. All rights reserved.

Chemical References

• MED30 protein, human
• Mediator Complex

Topics

• Adult
• Aged
• Aged, 80 and over
• Carcinoma, Renal Cell (genetics, pathology)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Disease Progression
• Disease-Free Survival
• Female
• Gene Knockdown Techniques
• Humans
• Immunohistochemistry
• Kidney Neoplasms (genetics, pathology)
• Male
• Mediator Complex (genetics, metabolism)
• Middle Aged
• Neoplasm Invasiveness (genetics)
• Survival Analysis
• Tissue Array Analysis