Analysis of cytomegalovirus-specific T-cell responses in patients with hypertension: comparison of assay methods and antigens.

Abstract

BACKGROUND:

Recent studies suggest an association between cytomegalovirus (CMV) infection and hypertension. In the present study, we used a variety of antigens and different assay methods to investigate the relationship between CMV-specific T-cell responses and arterial stiffness in patients with hypertension.

METHODS:

To evaluate arterial stiffness, pulse wave velocity (PWV) was measured in 207 hypertensive patients (average age, 63 ± 8 years). To measure CMV pp65 and IE-1-specific T-cell responses, we performed intracellular cytokine staining (ICS) and enzyme-linked immunospot (ELISPOT) assays. We also analyzed CMV-specific T-cell responses against 10 different CMV antigens using ELISPOT assays.

RESULTS:

In patients with hypertension, senescent CD8+ T-cell frequencies were significantly correlated with arterial stiffness. Moreover, arterial stiffness was independently associated with CMV pp65-specific CD8+ T-cell responses as measured by ICS. CMV-specific CD8+ T-cell responses measured by ICS and ELISPOT assays showed good agreement and significant correlation with each other. ELISPOT analyses against 10 different CMV antigens revealed a consistent response pattern irrespective of age, gender, and diabetes.

CONCLUSIONS:

CMV pp65-specific CD8+ T-cell responses were independently correlated with arterial stiffness in patients with hypertension. Additionally, the results of ICS and ELISPOT assays showed a significant correlation and good agreement with each other. These findings are important for guiding choices regarding the broad clinical application of CMV-specific T-cell response assays in this patient population.

• Authors: Jong-Chan Youn, Jun Yong Kim, Min Kyung Jung, Hee Tae Yu, Su-Hyung Park, In-Cheol Kim, Sun Ki Lee, Suk-Won Choi, Seongwoo Han, Kyu-Hyung Ryu, Sungha Park, Eui-Cheol Shin
• Journal: Clinical hypertension (Clin Hypertens) Vol. 24 Pg. 5 ( 2018) ISSN: 2056-5909 [Print] England
• PMID: 29568571 (Publication Type: Journal Article)