Evaluation of Potential Mechanisms Controlling the Catalase Expression in Breast Cancer Cells.

Abstract

Development of cancer cell resistance against prooxidant drugs limits its potential clinical use. MCF-7 breast cancer cells chronically exposed to ascorbate/menadione became resistant (Resox cells) by increasing mainly catalase activity. Since catalase appears as an anticancer target, the elucidation of mechanisms regulating its expression is an important issue. In MCF-7 and Resox cells, karyotype analysis showed that chromosome 11 is not altered compared to healthy mammary epithelial cells. The genomic gain of catalase locus observed in MCF-7 and Resox cells cannot explain the differential catalase expression. Since ROS cause DNA lesions, the activation of DNA damage signaling pathways may influence catalase expression. However, none of the related proteins (i.e., p53, ChK) was activated in Resox cells compared to MCF-7. The c-abl kinase may lead to catalase protein degradation via posttranslational modifications, but neither ubiquitination nor phosphorylation of catalase was detected after catalase immunoprecipitation. Catalase mRNA levels did not decrease after actinomycin D treatment in both cell lines. DNMT inhibitor (5-aza-2'-deoxycytidine) increased catalase protein level in MCF-7 and its resistance to prooxidant drugs. In line with our previous report, chromatin remodeling appears as the main regulator of catalase expression in breast cancer after chronic exposure to an oxidative stress.

• Authors: Christophe Glorieux, Juan Marcelo Sandoval, Nicolas Dejeans, Sandrine Nonckreman, Khadija Bahloula, Hélène A Poirel, Pedro Buc Calderon
• Journal: Oxidative medicine and cellular longevity (Oxid Med Cell Longev) Vol. 2018 Pg. 5351967 ( 2018) ISSN: 1942-0994 [Electronic] United States
• PMID: 29535798 (Publication Type: Journal Article)

Chemical References

• Catalase

Topics

• Breast Neoplasms (enzymology)
• Catalase (biosynthesis)
• Chromatin Assembly and Disassembly (physiology)
• Drug Resistance, Neoplasm (physiology)
• Female
• Gene Expression Regulation, Neoplastic (physiology)
• Humans
• MCF-7 Cells
• Oxidative Stress (physiology)