Viola Yedoensis Suppresses Cell Invasion by Targeting the Protease and NF-κB Activities in A549 and Lewis Lung Carcinoma Cells.

Abstract

Cancer metastasis is a vital trait in malignancies with complicated early diagnosis and therapeutic management. Therefore, the development of new remedies and the utilization of natural medicines that target metastasis are of great interest and have been studied extensively. Chinese medicinal herbs have various anti-carcinogenesis properties; however, the in vitro effect and mechanism of Viola yedoensis on cancer cell metastasis remains poorly understood. V. yedoensis extracts (VYE) can suppress the invasion of a highly metastatic human lung cancer cell line, A549 cells. According to gelatin zymography and casein zymography assays, VYE inhibited the activities of matrix metalloproteinases (MMPs) and urokinase-type plasminogen activator (u-PA). The results of reverse transcription-polymerase chain reaction and Western blotting revealed that VYE can alter the expression of proteinase inhibitor. VYE also suppressed the DNA binding activity of nuclear factor-kappa B. We concluded that VYE may inhibit tumor invasion by suppressing the activities of MMP and u-PA in lung cancer cells.

• Authors: She-Fang Huang, Shu-Chen Chu, Yi-Hsien Hsieh, Pei-Ni Chen, Yih-Shou Hsieh
• Journal: International journal of medical sciences (Int J Med Sci) Vol. 15 Issue 4 Pg. 280-290 ( 2018) ISSN: 1449-1907 [Electronic] Australia
• PMID: 29511364 (Publication Type: Journal Article)

Chemical References

• Drugs, Chinese Herbal
• viola yedoensis compound
• Peptide Hydrolases
• Urokinase-Type Plasminogen Activator
• Matrix Metalloproteinases

Topics

• A549 Cells
• Animals
• Carcinoma, Lewis Lung (drug therapy, genetics, pathology)
• Drugs, Chinese Herbal (administration & dosage, chemistry)
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Lung Neoplasms (drug therapy, genetics, pathology)
• Matrix Metalloproteinases (genetics)
• Neoplasm Invasiveness (genetics, pathology)
• Neoplasm Metastasis
• Peptide Hydrolases (genetics)
• Rats
• Urokinase-Type Plasminogen Activator (genetics)