[Effect of Electroacupuncture Stimulation at "Dazhui" (GV 14) and "Mingmen"(GV 4) on Cell Apoptosis of Spinal Cord and Expression of JNK Signaling Related Protein in Spinal Cord Injury Rats].

Abstract

OBJECTIVE:

To observe the effect of electroacupuncture (EA) stimulation at "Dazhui" (GV 14) and "Mingmen" (GV 4) of the Governor Vessel at different time-points on spinal cord neuronal apoptosis and the expression of c-Jun N-terminal kinases (JNK) protein in spinal cord injury (SCI) rats, so as to reveal its mechanism underlying improving SCI.

METHODS:

A total of 108 male SD rats were randomly divided into normal control, SCI model and EA groups which were further divided into 1, 3 and 7 d subgroups (12 rats/subgroup, 6 rats in each subgroup for TUNEL or Western blot, separately). SCI model was established by using the modified Allen's method. EA was applied to GV 14 and GV 4 for 20 min, once daily, for 1, 3 and 7 days, respectively. Basso-Beattie-Bresnahan (BBB) scale was adopted to assess the locomotor function of rats, the TUNEL method was used to examine neuronal apoptosis of injuried spinal cord, and the expression of phosphorylated (p)-c-Jun protein of T9-T11 spinal cord was detected by using Western blot.

RESULTS:

After modeling, the BBB scores of SCI rats on day 1, 3 and 7 were signi-ficantly decreased (P<0.01), while the numbers of apoptotic neuronal cells and the expression levels of p-c-Jun protein in the spinal cord were considerably increased at the 3 time-points in the model group (P<0.01, P<0.05). Following EA intervention, the decreased BBB scores on day 3 and 7, and the increased numbers of apoptotic neuronal cells on day 1, 3 and 7 and the up-regulated expression levels of p-c-Jun protein on day 3 and 7 were obviously suppressed (P<0.05, P<0.01).

CONCLUSIONS:

EA intervention can improve the locomotor function of SCI rats, which Feb be related to its effects in reducing neuronal apoptosis and down-regulating p-c-Jun protein in the injuried spinal cord.

• Authors: Wei Lv, Bing Li, Quan-Kai Jing, Yu-Ping Mo, Hai-Jiang Yao, Liang-Yu Song, Xin Wang, Ying-Qiu Mao, Zhi-Gang Li, Su-Hua Shi
• Journal: Zhen ci yan jiu = Acupuncture research (Zhen Ci Yan Jiu) Vol. 42 Issue 1 Pg. 14-9 (Feb 25 2017) ISSN: 1000-0607 [Print] China
• PMID: 29071992 (Publication Type: Journal Article)

Chemical References

• Proto-Oncogene Proteins c-jun

Topics

• Acupuncture Points
• Animals
• Apoptosis
• Electroacupuncture
• Humans
• MAP Kinase Signaling System
• Male
• Proto-Oncogene Proteins c-jun (genetics, metabolism)
• Rats
• Rats, Sprague-Dawley
• Spinal Cord (cytology, enzymology)
• Spinal Cord Injuries (enzymology, genetics, physiopathology, therapy)