Abstract |
Recent zoonotic outbreaks, such as Zika, Middle East respiratory syndrome and Ebola, have highlighted the need for rapid and accurate diagnostic assays that can be used to aid pathogen control. Q fever is a zoonotic disease caused by the transmission of Coxiella burnetii that can cause serious illness in humans through aerosols and is considered a potential bioterrorism agent. However, the existing assays are not suitable for the detection of this pathogen due to its low levels in real samples. We here describe a rapid bio-optical sensor for the accurate detection of Q fever and validate its clinical utility. By combining a bio-optical sensor, that transduces the presence of the target DNA based on binding-induced changes in the refractive index on the waveguide surface in a label-free and real-time manner, with isothermal DNA amplification, this new diagnostic tool offers a rapid (<20 min), 1-step DNA amplification/detection method. We confirmed the clinical sensitivity (>90%) of the bio-optical sensor by detecting C. burnetii in 11 formalin-fixed, paraffin-embedded liver biopsy samples from acute Q fever hepatitis patients and in 16 blood plasma samples from patients in which Q fever is the cause of fever of unknown origin.
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Authors | Bonhan Koo, Choong Eun Jin, Se Yoon Park, Tae Yoon Lee, Jeonghun Nam, Young-Rock Jang, Sun Mi Kim, Ji Yeun Kim, Sung-Han Kim, Yong Shin |
Journal | Journal of biophotonics
(J Biophotonics)
Vol. 11
Issue 4
Pg. e201700167
(04 2018)
ISSN: 1864-0648 [Electronic] Germany |
PMID | 29024445
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Topics |
- Biosensing Techniques
(instrumentation)
- Coxiella burnetii
(genetics, isolation & purification, physiology)
- Fever of Unknown Origin
(blood)
- Hepatitis
(diagnosis)
- Humans
- Optical Devices
- Polymerase Chain Reaction
- Q Fever
(diagnosis, microbiology)
- Time Factors
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