Abstract |
MicroRNAs ( miRNAs) are short noncoding RNAs that play critical roles in human malignancies and can be used as biomarkers for cancer. Until now, a number of biomarkers for prognosis of glioblastoma (GBM) have been reported in tumor tissues but only a few biomarkers in circulating fluid. Using a custom microarray, we previously identified 19 differentially expressed miRNAs in serum of patients with GBM. In this study, we investigated whether 3 of the 19 miRNAs in serum could be used as prognostic biomarkers for patients with GBM. We first validated the serum levels of 3 candidate miRNAs in an independent cohort of 24 GBM patients and 12 healthy volunteers by real-time quantitative reverse transcription PCR (qRT-PCR), and then evaluated the prognostic value of these miRNAs in a total of 36 GBM patients. The results show that the serum levels of the 3 miRNAs (miR-451a, miR-485-3p and miR-4298) determined by qRT-PCR are significantly different between 24 GBM patients and 12 healthy volunteers (all P <0.05) and are in concordance with the results of microarray analysis. High serum level of miR-451a is correlated with positive tumor O(6)-methylguanine-DNA methyltransferase (MGMT) expression (P = 0.040). Survival analysis showed that low serum miR-485-3p level is associated with poor progression-free survival (PFS) (P < 0.004) and overall survival (OS) (P < 0.023). Furthermore, univariate and multivariate Cox analyses demonstrated that that serum miR-485-3p expression is a significant independent prognostic factor for PFS and OS in GBM patients. In conclusion, serum miR-485-3p level is reduced and might be a potential prognostic biomarker in GBM patients.
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Authors | Zhi-Qiang Wang, Mei-Yin Zhang, Mei-Ling Deng, Nuo-Qing Weng, Hui-Yun Wang, Shao-Xiong Wu |
Journal | PloS one
(PLoS One)
Vol. 12
Issue 9
Pg. e0184969
( 2017)
ISSN: 1932-6203 [Electronic] United States |
PMID | 28931080
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- MIRN485 microRNA, human
- MicroRNAs
- Tumor Suppressor Proteins
- DNA Modification Methylases
- MGMT protein, human
- DNA Repair Enzymes
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Topics |
- Adolescent
- Adult
- Aged
- Biomarkers, Tumor
(blood, genetics)
- Brain Neoplasms
(blood, genetics, mortality, pathology)
- Case-Control Studies
- Combined Modality Therapy
- DNA Modification Methylases
(blood)
- DNA Repair Enzymes
(blood)
- Female
- Follow-Up Studies
- Gene Expression Regulation, Neoplastic
- Glioblastoma
(blood, genetics, mortality, pathology)
- Humans
- Male
- MicroRNAs
(blood, genetics)
- Middle Aged
- Neoplasm Staging
- Prognosis
- Real-Time Polymerase Chain Reaction
- Survival Rate
- Tumor Suppressor Proteins
(blood)
- Young Adult
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