[Changes of urinary proteins in a bacterial meningitis rat model].

Abstract

Unlike cerebrospinal fluid or blood, urine accumulates metabolic changes of the body and has the potential to be a promising source of early biomarkers discovery. Bacterial meningitis is a major cause of illness among neonates and children worldwide. In this study, we used Escherichia coli-injected rat model to mimic meningitis and collected urine samples on day 1 and day 3. We used two different methods to digest proteins and analyzed peptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). We identified 17 and 20 differential proteins by two methods respectively on day 1, and 5 differential proteins by filter-aided digestion method on day 3. Finding these differential proteins laid a foundation to further explore biomarkers of bacterial meningitis.

• Authors: Yanying Ni, Fanshuang Zhang, Manxia An, Youhe Gao
• Journal: Sheng wu gong cheng xue bao = Chinese journal of biotechnology (Sheng Wu Gong Cheng Xue Bao) Vol. 33 Issue 7 Pg. 1145-1157 (Jul 25 2017) ISSN: 1000-3061 [Print] China
• PMID: 28869734 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• Proteome

Topics

• Animals
• Biomarkers (urine)
• Chromatography, Liquid
• Meningitis, Escherichia coli (urine)
• Proteome
• Rats
• Tandem Mass Spectrometry
• Urinalysis