Molecular Recognition Profiles and Clinical Patterns of PR-10 Sensitization in a Birch-Free Mediterranean Area.

Abstract

BACKGROUND:

The order Fagales represents an important cause of tree-pollen allergy in northern countries. We investigated the IgE recognition profiles, mutual relationships, and association with clinical symptoms of a panel of allergens belonging to the PR-10 family, the main proteins responsible for Fagales allergy (Act d 8, Aln g 1, Api g 1, Ara h 8, Bet v 1, Cor a 1.0101, Cor a 1.0401, Gly m 4, Mal d 1, and Pru p 1).

METHODS:

A total of 526 PR-10-reactive subjects living in central and southern Italy were studied by ImmunoCAP-ISAC-112 microarray analysis.

RESULTS:

Overall, Bet v 1 reactivity was the most commonly (74%) observed among PR-10 proteins, but Cor a 1.0101 was the most prevalent in participants aged <6 years, and between 15 and 65 years. Overall, 26% of the PR-10-reactive persons were Bet v 1 negative, whilst 93.6% of the PR-10 polyreactive individuals were Bet v 1 positive. Among the 10 PR-10s evaluated, 100 combinations were recorded. The strongest association was observed between molecules with the highest sequence identities (Bet v 1 and Cor a 1.0101, Cor a 1.0401 or Aln g 1; Mal d 1 and Pru p 1). Bet v 1-, Cor a 1.0101-, and Aln g 1-specific IgE recognition was associated with respiratory symptoms, whilst Ara h 8, Cor a 1.0401, Gly m 4, Mal d 1, and Pru p 1 were selectively linked to an oral allergic syndrome.

CONCLUSIONS:

Testing IgE reactivity to a panel of PR-10s in a birch-free area discloses peculiar relationships between clinical phenotypes and sensitization profiles, allowing the identification of novel cluster patterns.

• Authors: Enrico Scala, Damiano Abeni, Lorenzo Cecchi, Emma Cristina Guerra, Maria Locanto, Lia Pirrotta, Mauro Giani, Riccardo Asero
• Journal: International archives of allergy and immunology (Int Arch Allergy Immunol) Vol. 173 Issue 3 Pg. 138-146 ( 2017) ISSN: 1423-0097 [Electronic] Switzerland
• PMID: 28787736 (Publication Type: Journal Article)
• Copyright: © 2017 S. Karger AG, Basel.

Chemical References

• Allergens
• Antigens, Plant
• Plant Proteins
• Immunoglobulin E

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Allergens (immunology)
• Antigens, Plant (immunology)
• Betula
• Child
• Child, Preschool
• Female
• Humans
• Hypersensitivity (blood, diagnosis)
• Immunoglobulin E (blood, immunology)
• Infant
• Italy
• Male
• Middle Aged
• Plant Proteins (immunology)
• Young Adult