Phytochemical-rich diets are protective against
chronic diseases and mediate their protective effect by regulation of oxidative stress (OS). However, it is proposed that under some circumstances,
phytochemicals can promote production of
reactive oxygen species (ROS) in vitro, which might drive OS-mediated signalling. Here, we investigated the effects of administering single doses of extracts of red cabbage and grape skin to pigs. Blood samples taken at baseline and 30 min intervals for 4 hours following intake were analyzed by measures of
antioxidant status in plasma, including
Trolox equivalent
antioxidant capacity (TEAC) and
glutathione peroxidase (GPx) activity. In addition, dose-dependent production of
hydrogen peroxide (H₂O₂) by the same extracts was measured in untreated commercial pig plasma in vitro. Plasma from treated pigs showed extract dose-dependent increases in non-enzymatic (plasma TEAC) and enzymatic (GPx)
antioxidant capacities. Similarly, extract dose-dependent increases in H₂O₂ were observed in commercial pig plasma in vitro. The
antioxidant responses to extracts by treated pigs were highly correlated with their respective yields of H₂O₂ production in vitro. These results support that
dietary phytochemicals regulate OS via direct and indirect
antioxidant mechanisms. The latter may be attributed to the ability to produce H₂O₂ and to thereby stimulate cellular
antioxidant defence systems.