Immunohistochemical expression of TGF-β1 and MMP-9 in periapical lesions.

Abstract

The objective of this study was to evaluate the expression of matrix metalloproteinase 9 (MMP-9) and transforming growth factor beta (TGF-β1) in periapical lesion samples correlated with the intensity of the inflammatory infiltrate and thickness of the epithelial lining. Forty-five cases of periapical lesions (23 periapical granulomas and 22 radicular cysts) were subjected to morphological and immunohistochemical analyses using anti-MMP-9 and anti-TGF-β1 antibodies. The data were analyzed using the following tests: non-parametric Mann-Whitney, chi-square, Fisher's exact test and Spearman's correlation test (P<0.05). Analysis of inflammatory infiltrate revealed that 78% of periapical granulomas presented infiltrate grade III, in contrast with 32% of radicular cysts (P<0.001). Morphological evaluation of the epithelial thickness in radicular cysts revealed the presence of atrophic epithelium in 86% of the cysts. The immunostaining of MMP-9 was score 2 in 67% of the granulomas and 77% of the cysts. Both lesions were predominantly score 1 for TGF-β1. Significant differences were confirmed between the expression scores of TGF-β1 and MMP-9 in periapical granulomas (p = 0.004) and in radicular cysts (p < 0.001). Expression of TGF-β1 was different for periapical granulomas and radicular cysts. This immunoregulatory cytokine seems more representative in asymptomatic lesions. The extracellular matrix remodeling process dependent on MMP-9 seems to be similar for both periapical granulomas and radicular cysts. TGF-β1 and MMP-9 may play an important role in the maintenance of periapical lesions.

• Authors: Pâmella Recco Álvares, José Alcides Almeida de Arruda, Leorik Pereira da Silva, George João Ferreira do Nascimento, Maria Fonseca da Silveira, Ana Paula Veras Sobral
• Journal: Brazilian oral research (Braz Oral Res) Vol. 31 Pg. e51 (Jul 03 2017) ISSN: 1807-3107 [Electronic] Brazil
• PMID: 28678970 (Publication Type: Journal Article)

Chemical References

• Transforming Growth Factor beta1
• Matrix Metalloproteinase 9

Topics

• Adult
• Biopsy
• Epithelial Cells (pathology)
• Female
• Humans
• Immunohistochemistry (methods)
• Male
• Matrix Metalloproteinase 9 (analysis)
• Periapical Granuloma (immunology, metabolism, pathology)
• Radicular Cyst (chemistry, immunology, pathology)
• Severity of Illness Index
• Statistics, Nonparametric
• Transforming Growth Factor beta1 (analysis)