Alterations of expression of inflammation/immune-related genes in the dorsal and ventral striatum of adult C57BL/6J mice following chronic oxycodone self-administration: a RNA sequencing study.

Abstract

INTRODUCTION:

Non-medical use of prescription opioids such as the mu opioid receptor (MOP-r) agonist oxycodone is a growing problem in the USA and elsewhere. There is limited information about oxycodone's impact on diverse gene systems in the brain.

OBJECTIVES:

The current study was designed to examine how chronic oxycodone self-administration (SA) affects gene expression in the terminal areas of the nigrostriatal and mesolimbic dopaminergic pathways in mice.

METHOD:

Adult male C57BL/6J mice underwent a 14-day oxycodone self-administration procedure (4 h/day, 0.25 mg/kg/infusion, FR1) and were euthanized 1 h after the last session. The dorsal and ventral striata were dissected, and total RNAs were extracted. Gene expressions were examined using RNA sequencing.

RESULT:

We found that oxycodone self-administration exposure led to alterations of expression in numerous genes related to inflammation/immune functions in the dorsal striatum (54 upregulated genes and 1 downregulated gene) and ventral striatum (126 upregulated genes and 15 downregulated genes), with 38 upregulated genes identified in both brain regions.

CONCLUSION:

This study reveals novel neurobiological mechanisms underlying some of the effects of a commonly abused prescription opioid. We propose that inflammation/immune gene systems may undergo a major change during chronic self-administration of oxycodone.

• Authors: Yong Zhang, Yupu Liang, Orna Levran, Matthew Randesi, Vadim Yuferov, Connie Zhao, Mary Jeanne Kreek
• Journal: Psychopharmacology (Psychopharmacology (Berl)) Vol. 234 Issue 15 Pg. 2259-2275 (Aug 2017) ISSN: 1432-2072 [Electronic] Germany
• PMID: 28653080 (Publication Type: Journal Article)

Chemical References

• Analgesics, Opioid
• Inflammation Mediators
• Oxycodone
• Dopamine

Topics

• Age Factors
• Analgesics, Opioid (administration & dosage)
• Animals
• Dopamine
• Gene Expression (drug effects, physiology)
• Inflammation (genetics, immunology, metabolism)
• Inflammation Mediators (immunology, metabolism)
• Male
• Mice
• Mice, Inbred C57BL
• Oxycodone (administration & dosage)
• Self Administration
• Sequence Analysis, RNA (methods)
• Ventral Striatum (drug effects, immunology, metabolism)