Abstract | OBJECTIVES: METHODS: A total of 100 C. albicans strains isolated from women with vulvovaginal candidiasis referred to Shiraz medical clinics were enrolled in the study. All the isolates were primarily identified by conventional methods. PCR-RFLP method was used for the confirmation and identification of C. albicans and C. dubliniensis. Moreover, in vitro proteinase and coagulase activities of these isolates were evaluated using bovine serum albumin media and classical rabbit plasma tube test. RESULTS: As a result, PCR-RFLP identified 100% of the isolates as C. albicans, and no C. dubliniensis could be identified in this study. 84% of the isolates showed proteinase activity, whereas coagulase activity was only detected in 5% of the isolates. CONCLUSIONS: This study reveals that C. dubliniensis plays no role in vaginal candidiasis in Iranian patients. Proteinase production could be an essential virulence factor in C. albicans pathogenicity, but coagulase activity has less potential in this matter.
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Authors | Maryam Jafari, Samira Salari, Keyvan Pakshir, Kamiar Zomorodian |
Journal | Microbial pathogenesis
(Microb Pathog)
Vol. 110
Pg. 73-77
(Sep 2017)
ISSN: 1096-1208 [Electronic] England |
PMID | 28642006
(Publication Type: Journal Article)
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Copyright | Copyright © 2017 Elsevier Ltd. All rights reserved. |
Chemical References |
- Coagulase
- DNA, Fungal
- Peptide Hydrolases
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Topics |
- Candida
(enzymology, genetics, isolation & purification, pathogenicity)
- Candida albicans
(enzymology, genetics, isolation & purification, pathogenicity)
- Candidiasis, Vulvovaginal
(diagnosis, microbiology)
- Coagulase
(analysis)
- DNA, Fungal
(isolation & purification)
- Female
- Humans
- Iran
- Peptide Hydrolases
(analysis)
- Polymerase Chain Reaction
(methods)
- Polymorphism, Restriction Fragment Length
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