Influence of aspirin on the CX3CL1/CX3CR1 signaling pathway in acute pulmonary embolism.

Abstract

The present study aimed to explore the influence of aspirin on the CX3CL1/CX3CR1 signaling pathway in acute pulmonary embolism (APE) in rats. Our previous study found that CX3CL1/CX3CR1 was increased in APE. However, the effect of this signaling pathway on APE remains unclear. CX3CL1-shRNA adenovirus and CX3CL1-overexpression vector were constructed. Male Sprague-Dawley rats were randomly divided into 9 groups (n=10): normal group (group N), sham operation group (group Sham), sham operation + aspirin group (group ASP), model group (group M), model + ASP group (group M+A), model + shRNA group (group M+SH), sham operation + CX3CL1-overexpression vector group (group Sham+Cx3), model + ASP + shRNA group (group M+A+SH), and model + ASP + CX3CL1-overexpression vector group (group M+A+CX3). Arterial pressure detection, hematoxylin and eosin staining, reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay, and laser confocal scanning microscopy were applied. Aspirin significantly decreased pulmonary artery pressure, improve pathological changes in the embolism, and decreased the expression of CX3CL1/CX3CR1 and CX3CL1/NF-κB. Moreover, the adenovirus-overexpression CX3CL1 vector aggravated the inflammatory changes in APE, which were improved by aspirin. However, the intervention of the adenovirus CX3CL1 vector reduced the change, while its combination with aspirin significantly improved the change. In conclusion, aspirin improved pathological changes in rats with APE via the CX3CL1/CX3CR1 signaling pathway.

• Authors: Zhirong Zhang, Weiji Yang, Rongbiao Ying, Ying Shi, Huifang Jiang, Danli Cai, Jing Kuang, Ruhui Yang, Lingcong Wang
• Journal: International journal of molecular medicine (Int J Mol Med) Vol. 39 Issue 6 Pg. 1580-1588 (Jun 2017) ISSN: 1791-244X [Electronic] Greece
• PMID: 28487961 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• CX3C Chemokine Receptor 1
• CX3CR1 protein, rat
• Chemokine CX3CL1
• Cx3cl1 protein, rat
• NF-kappa B
• Aspirin

Topics

• Acute Disease
• Animals
• Anti-Inflammatory Agents, Non-Steroidal (therapeutic use)
• Aspirin (therapeutic use)
• CX3C Chemokine Receptor 1 (analysis, immunology)
• Chemokine CX3CL1 (analysis, immunology)
• Male
• NF-kappa B (analysis, immunology)
• Pulmonary Embolism (drug therapy, immunology, pathology)
• Rats, Sprague-Dawley
• Signal Transduction (drug effects)