Abstract |
Rheumatoid arthritis (RA) is an autoimmune disease with an unknown etiology, occurring in approximately 1.0% of general population. More and more studies have suggested that long non-coding RNAs (lncRNAs) could play important roles in various biological processes and be associated with the pathogenesis of different kinds of diseases including RA. Although a large number of lncRNAs have been found, our knowledge of their function and physiological/pathological significance is still in its infancy. In order to reveal functional lncRNAs and identify the key lncRNAs in RA, we reconstructed a global triple network based on the competitive endogenous RNA ( ceRNA) theory using the data from National Center for Biotechnology Information Gene Expression Omnibus and our previous paper. Meanwhile, Gene Ontology (GO) and pathway analysis were performed using Cytoscape plug-in BinGO and Database for Annotation, Visualization, and Integration Discovery (DAVID), respectively. We found that the lncRNA- miRNA- mRNA network was composed of 7 lncRNA nodes, 90 mRNA nodes, 24 miRNA nodes, and 301 edges. The functional assay showed that 147 GO terms and 23 pathways were enriched. In addition, three lncRNAs (S5645.1, XR_006437.1, J01878) were highly related to RA, and therefore, were selected as key lncRNAs. This study suggests that specific lncRNAs are associated with the development of RA, and three lncRNAs (S5645.1, XR_006437.1, J01878) could be used as potential diagnostic biomarkers and therapeutic targets.
|
Authors | Hui Jiang, Rong Ma, Shubiao Zou, Yongzhong Wang, Zhuqing Li, Weiping Li |
Journal | Molecular bioSystems
(Mol Biosyst)
Vol. 13
Issue 6
Pg. 1182-1192
(Jun 01 2017)
ISSN: 1742-2051 [Electronic] England |
PMID | 28470264
(Publication Type: Journal Article)
|
Chemical References |
- MicroRNAs
- RNA, Long Noncoding
- RNA, Messenger
|
Topics |
- Arthritis, Rheumatoid
(genetics, metabolism)
- Gene Expression Profiling
- Gene Expression Regulation, Neoplastic
(genetics)
- Gene Ontology
- Humans
- MicroRNAs
(metabolism)
- Oligonucleotide Array Sequence Analysis
- RNA, Long Noncoding
(metabolism)
- RNA, Messenger
(metabolism)
|