RNA interference (RNAi) has a great promise in treating various acquired and
hereditary diseases. However, it remains highly desirable to develop new delivery system to circumvent complex extra- and intracellular barriers for successful clinical translation. Here, we report on a versatile polymeric vector, bioreducible fluorinated
peptide dendrimers (BFPD), for efficient and safe
small interfering RNA (
siRNA) delivery. In virtue of skillfully integrating all of the unique advantages of reversible cross-linking, fluorination, and
peptide dendrimers, this novel vector can surmount almost all extra- and intracellular barriers associated with local
siRNA delivery through highly improved physiological stability and serum resistance, significantly increased intratumoral enrichment, cellular internalization, successful facilitation of endosomal escape, and cytosolic
siRNA release. BFPD polyplexes, carrying small interfering
vascular endothelial growth factor (siVEGF), demonstrated excellent
VEGF silencing efficacy (∼65%) and a strong capability for inhibiting HeLa cell proliferation. More importantly, these polyplexes showed superior performance in long-term enrichment in the
tumor sites and had a high level of
tumor growth inhibition. Furthermore, these polyplexes not only exhibited excellent in vivo antitumor efficacy but also demonstrated superior biocompatibility, compared with LPF2000, both in vivo and in vitro. These findings indicate that BFPD is an efficient and safe
siRNA delivery system and has remarkable potential for RNAi-based
cancer treatment.