Abstract |
Long intergenic noncoding RNAs ( lincRNAs) play important roles in regulating the biological functions and underlying molecular mechanisms of glioma. Here, we investigated the expression level and biological function of linc-OIP5 in glioma. In the current study, we used quantitative real-time polymerase chain reaction (qRT-PCR) to determine the expression of linc-OIP5 in glioma tissues and in adjacent normal tissues. Level of linc-OIP5 was up-regulated in glioma tissues and significantly correlated with the advanced tumor stage (III/IV). Subsequently, the efficacy of knockdown of linc-OIP5 by linc-OIP5-small interfering RNA ( siRNA) was evaluated in vitro, and we found that knockdown of linc-OIP5 can inhibit glioma cells proliferation, migration in vitro and tumor formation in vivo. Further mechanistic studies revealed the effect of linc-OIP5 knockdown on glioma cell phenotype at least partially through down-regulation of YAP and inhibition of Notch signaling pathway activity. Thus, our study provides evidence that linc-OIP5 is a potential therapeutic target and novel molecular biomarker for glioma.
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Authors | Guo-Wen Hu, Lei Wu, Wei Kuang, Yong Chen, Xin-Gen Zhu, Hua Guo, Hai-Li Lang |
Journal | Gene
(Gene)
Vol. 610
Pg. 24-31
(Apr 30 2017)
ISSN: 1879-0038 [Electronic] Netherlands |
PMID | 28189759
(Publication Type: Journal Article)
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Copyright | Copyright © 2017. Published by Elsevier B.V. |
Chemical References |
- Cell Cycle Proteins
- Chromosomal Proteins, Non-Histone
- OIP5 protein, human
- RNA, Long Noncoding
- long noncoding RNA OIP5, human
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Topics |
- Animals
- Cell Cycle Proteins
- Cell Line, Tumor
- Cell Movement
- Cell Proliferation
- Central Nervous System Neoplasms
(genetics, metabolism)
- Chromosomal Proteins, Non-Histone
(genetics)
- Down-Regulation
- Female
- Gene Knockdown Techniques
- Glioma
(genetics, metabolism)
- Humans
- Male
- Mice
- Mice, Nude
- Middle Aged
- RNA, Long Noncoding
(genetics, metabolism)
- Retrospective Studies
- Signal Transduction
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