Abstract | BACKGROUND: OBJECTIVES: DESIGN: In a randomized, double-blind, placebo-controlled trial (the Dietary Intervention, Stem cells and Colorectal Cancer (DISC) Study), 75 healthy participants were supplemented with RS and/or polydextrose or placebo for 50 d in a 2 × 2 factorial design. Pre- and postintervention stool samples and rectal mucosal biopsies were collected and used to quantify calprotectin and expression of 12 Wnt-related genes, respectively. The expression of 10 microRNAs predicted to target SFRP1 was also quantified by quantitative reverse transcriptase-polymerase chain reaction, and DNA methylation was quantified at 7 CpG sites within the SFRP1 promoter region by pyrosequencing. RESULTS: NDC supplementation did not affect fecal calprotectin concentration. SFRP1 mRNA expression was reduced by both RS (P = 0.005) and polydextrose (P = 0.053). RS and polydextrose did not affect SFRP1 methylation or alter the expression of 10 microRNAs predicted to target SFRP1. There were no significant interactions between RS and polydextrose. CONCLUSIONS: RS and polydextrose supplementation did not affect fecal calprotectin concentrations. Downregulation of SFRP1 with RS and polydextrose could result in increased Wnt pathway activity. However, effects on Wnt pathway activity and downstream functional effects in the healthy large-bowel mucosa remain to be investigated. The DISC Study was registered at clinicaltrials.gov as NCT01214681.
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Authors | Fiona C Malcomson, Naomi D Willis, Iain McCallum, Long Xie, Idoia Ibero-Baraibar, Wing C Leung, Seamus Kelly, D Michael Bradburn, Nigel J Belshaw, Ian T Johnson, John C Mathers |
Journal | The American journal of clinical nutrition
(Am J Clin Nutr)
Vol. 105
Issue 2
Pg. 400-410
(02 2017)
ISSN: 1938-3207 [Electronic] United States |
PMID | 28077379
(Publication Type: Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
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Chemical References |
- Dietary Carbohydrates
- Glucans
- Intercellular Signaling Peptides and Proteins
- Leukocyte L1 Antigen Complex
- Membrane Proteins
- MicroRNAs
- RNA, Messenger
- SFRP1 protein, human
- Starch
- polydextrose
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Topics |
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- DNA Methylation
- Dietary Carbohydrates
(administration & dosage, pharmacokinetics)
- Double-Blind Method
- Down-Regulation
- Epigenesis, Genetic
- Feces
(chemistry)
- Female
- Glucans
(chemistry)
- Humans
- Intercellular Signaling Peptides and Proteins
(genetics, metabolism)
- Intestinal Mucosa
(metabolism)
- Leukocyte L1 Antigen Complex
(chemistry)
- Male
- Membrane Proteins
(genetics, metabolism)
- MicroRNAs
(genetics, metabolism)
- Middle Aged
- Promoter Regions, Genetic
- RNA, Messenger
(genetics, metabolism)
- Starch
(chemistry)
- Wnt Signaling Pathway
- Young Adult
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