LncRNA-ANCR down-regulation suppresses invasion and migration of colorectal cancer cells by regulating EZH2 expression.

Abstract

Our study aimed to explore the effects of long noncoding RNA (lncRNA)-ANCR on the invasion and migration of colorectal cancer (CRC) cells by regulating enhancer of zeste homolog 2 (EZH2) expression. CRC tissues and adjacent normal tissues were collected and CRC SW620 cells line and normal human intestinal epithelial cells (HIECs) were incubated. CRC SW620 cells line was transfected with ANCR-siRNA. The expressions of ANCR and EZH2 mRNA were measured by real-time quantitative polymerase chain reaction (RT-qPCR). EZH2 and trimethylation of H3K27 (H3K27me3) protein expressions were detected using Western blotting. The relationship between ANCR and EZH2 was determined through RNA pull-down and co-immunoprecipitation (co-IP) assays. Cell invasion and migration were determined by Trans-well and cell scratch assays. ANCR, EZH2 and H3K27me3 expressions were up-regulated in CRC tissues and SW620 cells (all P < 0.05). After transfected with ANCR-siRNA, SW620 cells showed decreased ANCR expression and EZH2 mRNA and protein expressions (all P < 0.05). According to the results of RNA pull-down and co-IP assays, ANCR could specifically bind to EZH2. The results of Trans-well and cell scratch tests showed that when ANCR expression was decreased, the invasion and migration abilities of SW620 cells significantly declined (both P < 0.05). In conclusion, these results suggest that lncRNA-ANCR could influence the invasion and migration of CRC cells by specifically binding to EZH2.

• Authors: Zhao-Yang Yang, Fang Yang, Ying-Li Zhang, Bao Liu, Meng Wang, Xuan Hong, Yan Yu, Yao-Hui Zhou, Hai Zeng
• Journal: Cancer biomarkers : section A of Disease markers (Cancer Biomark) Vol. 18 Issue 1 Pg. 95-104 ( 2017) ISSN: 1875-8592 [Electronic] Netherlands
• PMID: 27983539 (Publication Type: Journal Article)

Chemical References

• ANCR long noncoding RNA, human
• RNA, Long Noncoding
• RNA, Messenger
• EZH2 protein, human
• Enhancer of Zeste Homolog 2 Protein

Topics

• Cell Line, Tumor
• Cell Movement (genetics)
• Colorectal Neoplasms (genetics, pathology)
• Down-Regulation
• Enhancer of Zeste Homolog 2 Protein (genetics)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Male
• Middle Aged
• Neoplasm Invasiveness
• RNA, Long Noncoding (biosynthesis, genetics)
• RNA, Messenger (biosynthesis, genetics)