HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Progressive development of endometriosis and its hindrance by anti-platelet treatment in mice with induced endometriosis.

Abstract
We have recently shown that platelets drive smooth muscle metaplasia (SMM) and fibrogenesis in endometriosis through epithelial-mesenchymal transition (EMT) and fibroblast-to-myofibroblast transdifferentiation (FMT). To see whether this is true in vivo, this prospective, randomized, and serially evaluated mouse investigation was conducted. Endometriosis was induced in female Balb/C mice, which were then randomly divided into two groups: Tanshinone IIA (TAN) and control (CTL) groups. TAN mice were treated with TAN but CTL mice received none. Every week until the 6th week after induction, five mice from each group were killed. Lesion weight was measured and lesion samples were subjected to immunohistochemistry and histochemistry analysis of platelet aggregation (CD41), E-cadherin, TGF-β1, phosphorylated Smad3, α-SMA, collagen I, CCN2, LOX, desmin and SM-MHC, and the extent of fibrosis was evaluated by Masson trichrome staining. It was found that endometriotic lesions exhibited progressive cellular changes consistent with the progressive EMT, FMT, SMM, and fibrogenesis. TAN treatment resulted in significant hindrance of EMT, FMT, SMM and fibrogenesis, and reduced lesion weight (all P-values <0.05). These data corroborate the notion that endometriotic lesions undergo progressive EMT and FMT, giving rise to SMM and ultimately fibrosis. This understanding sheds new light onto the natural history of endometriosis.
AuthorsQi Zhang, Xishi Liu, Sun-Wei Guo
JournalReproductive biomedicine online (Reprod Biomed Online) Vol. 34 Issue 2 Pg. 124-136 (Feb 2017) ISSN: 1472-6491 [Electronic] Netherlands
PMID27916451 (Publication Type: Journal Article)
CopyrightCopyright © 2016 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
Chemical References
  • Platelet Aggregation Inhibitors
Topics
  • Animals
  • Blood Platelets (cytology)
  • Cell Differentiation
  • Endometriosis (chemically induced, physiopathology)
  • Epithelial-Mesenchymal Transition
  • Female
  • Fibroblasts (cytology)
  • Metaplasia
  • Mice
  • Mice, Inbred BALB C
  • Muscle, Smooth (pathology)
  • Myofibroblasts (cytology)
  • Platelet Activation
  • Platelet Aggregation
  • Platelet Aggregation Inhibitors (chemistry)
  • Prospective Studies
  • Random Allocation

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: