Abstract |
This study was undertaken to determine if visfatin is involved in the inflammation or apoptosis introduced by LPS in rats. Forty 8-week old Wistar rats were divided into four groups (n=10 in each group) and injected with saline, visfatin, LPS and visfatin+LPS co-stimulated via caudal vein. The duodenum, jejunum and ileum were harvested from all the rats. Compared to the saline treated group, visfatin significantly increased the number of TUNEL-positive apoptotic cells and the expression of caspase-3 protein in intestinal mucosa. Similarly, ELISA and western blot analysis also showed the up-regulation of pro-caspase-3 and cleaved caspase-3 expression in the visfatin group compared to the control group. In contrast to LPS, visfatin down-regulated the expression of cleaved-caspase-3 in the visfatin+LPS co-stimulated group, resulting in a significant decrease in apoptosis in intestinal mucosal cells. We observed more pro-caspase-3 positive cells in the visfatin+LPS co-stimulated group. The results indicate that, in the presence of LPS, visfatin plays an important role in the regulation of cell apoptosis and inflammation.
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Authors | Ying Zhou, Huai-Rui Yuan, Lu Cui, Abdur Rahman Ansari, Ke Xiao, You Luo, Xin-Tong Wu, Liang Guo, Faheem Ahmed Khan, Zhi Yang, Hui Song |
Journal | Acta histochemica
(Acta Histochem)
Vol. 119
Issue 1
Pg. 26-31
(Jan 2017)
ISSN: 1618-0372 [Electronic] Germany |
PMID | 27884396
(Publication Type: Journal Article)
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Copyright | Copyright © 2016 Elsevier GmbH. All rights reserved. |
Chemical References |
- Drug Combinations
- Lipopolysaccharides
- Nicotinamide Phosphoribosyltransferase
- Casp3 protein, rat
- Caspase 3
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Topics |
- Animals
- Apoptosis
(drug effects)
- Caspase 3
(genetics, metabolism)
- Drug Combinations
- Duodenum
(cytology, drug effects, immunology)
- Epithelial Cells
(cytology, drug effects, immunology)
- Gene Expression
- Ileum
(cytology, drug effects, immunology)
- Immunity, Mucosal
(drug effects)
- In Situ Nick-End Labeling
- Intestinal Mucosa
(cytology, drug effects, immunology)
- Jejunum
(cytology, drug effects, immunology)
- Lipopolysaccharides
(pharmacology)
- Nicotinamide Phosphoribosyltransferase
(pharmacology)
- Rats
- Rats, Wistar
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