Claudin-1 correlates with poor prognosis in lung adenocarcinoma.

Abstract

BACKGROUND:

This study was conducted to investigate the clinical significance of claudin-1 (CLDN1) expression in patients with lung adenocarcinoma.

METHODS:

We examined CLDN1 protein expression by immunohistochemistry in a tissue microarray from 258 patients with lung adenocarcinoma. We investigated messenger ribonucleic acid (mRNA) expression in H358 (formerly bronchioloalveolar carcinoma) and lung adenocarcinoma cell lines (A549) by real-time reverse transcriptase-polymerase chain reaction.

RESULTS:

Multivariate analysis showed that prognostic factors for lung adenocarcinoma were histologic type, CLDN1, T stage and N stage. Patients with positive CLDN1 expression had a poorer prognosis than patients with negative CLDN1 expression. CLDN1 expression was correlated with Ras and epidermal growth factor receptor (EGFR) expression. Patients with positive expressions of both CLDN1 and Ras/EGFR had a poorer prognosis than patients with CLDN1 (+) Ras/EGFR(-) or CLDN1 (-) Ras/EGFR(+) and patients with negative expressions of both CLDN1 and Ras/EGFR. CLDN1 mRNA expression was lower in the H358 compared with the lung adenocarcinoma cell line (A549).

CONCLUSION:

The combination of CLDN1 and Ras/EGFR is a valuable independent prognostic predictor for lung adenocarcinoma.

• Authors: Bing-Sheng Sun, Yi-Qun Yao, Bao-Xiang Pei, Zhen-Fa Zhang, Chang-Li Wang
• Journal: Thoracic cancer (Thorac Cancer) Vol. 7 Issue 5 Pg. 556-563 (Sep 2016) ISSN: 1759-7714 [Electronic] Singapore
• PMID: 27766775 (Publication Type: Journal Article)
• Copyright: © 2016 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd.

Chemical References

• CLDN1 protein, human
• Claudin-1
• EGFR protein, human
• ErbB Receptors
• ras Proteins

Topics

• Adenocarcinoma (genetics, metabolism, pathology)
• Adenocarcinoma of Lung
• Cell Line, Tumor
• Claudin-1 (genetics, metabolism)
• ErbB Receptors (genetics)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Lung Neoplasms (genetics, metabolism, pathology)
• Male
• Neoplasm Staging
• Prognosis
• Tissue Array Analysis (methods)
• Up-Regulation
• ras Proteins (genetics)