Near infrared imaging-guided photodynamic therapy under an extremely low energy of light by galactose targeted amphiphilic polypeptide micelle encapsulating BODIPY-Br2.

Abstract

Near infrared (NIR) imaging-guided photodynamic therapy (PDT) is attractive, especially the utilization of one dye as both a photosensitizer and fluorescent probe, and the as-synthesized BODIPY-Br2 molecule is a candidate. Here, a galactose targeted amphiphilic copolymer of a polypeptide was synthesized and its micelles work as nanocarriers for BODIPY for targeting the NIR imaging-guided PDT of hepatoma cancer cells. At the same time, BODIPY could light up the cytoplasm for real-time imaging and kill cancer cells when the light was switched on. In vitro tests performed on both HepG2 and HeLa cells confirmed that the as-prepared PMAGP-POEGMA-PLys-B micelles showed efficient cell suppression of the cells with galactose receptors in the presence of light under an extremely low energy density (6.5 J cm-2). This protocol highlights the potential of polypeptides as biodegradable carriers for NIR image-guided and confined targeting photodynamic therapy.

• Authors: Le Liu, Zheng Ruan, Tuanwei Li, Pan Yuan, Lifeng Yan
• Journal: Biomaterials science (Biomater Sci) Vol. 4 Issue 11 Pg. 1638-1645 (Oct 18 2016) ISSN: 2047-4849 [Electronic] England
• PMID: 27709131 (Publication Type: Journal Article)

Chemical References

• 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
• Boron Compounds
• Micelles
• Peptides
• Galactose

Topics

• Boron Compounds (chemistry, metabolism)
• Galactose (chemistry)
• HeLa Cells
• Humans
• Infrared Rays
• Micelles
• Peptides (metabolism)
• Photochemotherapy (methods)