Melatonin modulates adiponectin expression on murine colitis with sleep deprivation.

Abstract

AIM:

To determine adiponectin expression in colonic tissue of murine colitis and systemic cytokine expression after melatonin treatments and sleep deprivation.

METHODS:

The following five groups of C57BL/6 mice were used in this study: (1) group I, control; (2) group II, 2% DSS induced colitis for 7 d; (3) group III, 2% DSS induced colitis and melatonin treatment; (4) group IV, 2% DSS induced colitis with sleep deprivation (SD) using specially designed and modified multiple platform water baths; and (5) group V, 2% DSS induced colitis with SD and melatonin treatment. Melatonin (10 mg/kg) or saline was intraperitoneally injected daily to mice for 4 d. The body weight was monitored daily. The degree of colitis was evaluated histologically after sacrificing the mice. Immunohistochemical staining and Western blot analysis was performed using anti-adiponectin antibody. After sampling by intracardiac punctures, levels of serum cytokines were measured by ELISA.

RESULTS:

Sleep deprivation in water bath exacerbated DSS induced colitis and worsened weight loss. Melatonin injection not only alleviated the severity of mucosal injury, but also helped survival during stressful condition. The expression level of adiponectin in mucosa was decreased in colitis, with the lowest level observed in colitis combined with sleep deprivation. Melatonin injection significantly (P < 0.05) recovered the expression of adiponectin. The expression levels of IL-6 and IL-17 were increased in the serum of mice with DSS colitis but decreased after melatonin injection.

CONCLUSION:

This study suggested that melatonin modulated adiponectin expression in colonic tissue and melatonin and adiponectin synergistically potentiated anti-inflammatory effects on colitis with sleep deprivation.

• Authors: Tae Kyun Kim, Young Sook Park, Haing-Woon Baik, Jin Hyun Jun, Eun Kyung Kim, Jae Woong Sull, Ho Joong Sung, Jin Woo Choi, Sook Hee Chung, Myung Chan Gye, Ju Yeon Lim, Jun Bong Kim, Seong Hwan Kim
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 22 Issue 33 Pg. 7559-68 (Sep 07 2016) ISSN: 2219-2840 [Electronic] United States
• PMID: 27672276 (Publication Type: Journal Article)

Chemical References

• Adiponectin
• Antibodies
• Cytokines
• Melatonin

Topics

• Adiponectin (metabolism)
• Animals
• Antibodies (chemistry)
• Body Weight
• Colitis (complications, metabolism)
• Cytokines (blood, metabolism)
• Enzyme-Linked Immunosorbent Assay
• Immunohistochemistry
• Inflammation
• Male
• Melatonin (metabolism)
• Mice
• Mice, Inbred C57BL
• Sleep Deprivation (complications, metabolism)