The utility of urine-circulating miRNAs for detection of prostate cancer.

Abstract

BACKGROUND:

In this paper, the utility of urine-circulating microRNAs (miRNAs) as the potential biomarker of prostate cancer (PCa), the second most prevalent male cancer worldwide, was evaluated.

METHODS:

Cancerous (N=56) and non-cancerous (N=16) prostate tissues were analysed on TaqMan Low Density Array, with the initial screening of 754 miRNAs in a subset of the samples. The abundance of selected miRNAs was analysed in urine specimens from two independent cohorts of patients with PCa (N=215 overall), benign prostatic hyperplasia (BPH; N=23), and asymptomatic controls (ASC; N=62) by means of quantitative reverse transcription PCR.

RESULTS:

Over 100 miRNAs were found deregulated in PCa as compared with non-cancerous prostate tissue. After thorough validation, four miRNAs were selected for the analysis in urine specimens. The abundance of miR-148a and miR-375 in urine was identified as specific biomarkers of PCa in both cohorts. Combined analysis of urine-circulating miR-148a and miR-375 was highly sensitive and specific for PCa in both cohorts (AUC=0.79 and 0.84) and strongly improved the diagnostic power of the PSA test (AUC=0.85, cohort PCa1), including the grey diagnostic zone (AUC=0.90).

CONCLUSIONS:

Quantitative measurement of urine-circulating miR-148a and miR-375 can serve as the non-invasive tool for sensitive and specific detection of PCa.

• Authors: Kristina Stuopelyte, Kristina Daniunaite, Arnas Bakavicius, Juozas R Lazutka, Feliksas Jankevicius, Sonata Jarmalaite
• Journal: British journal of cancer (Br J Cancer) Vol. 115 Issue 6 Pg. 707-15 (Sep 06 2016) ISSN: 1532-1827 [Electronic] England
• PMID: 27490805 (Publication Type: Clinical Trial, Journal Article)

Chemical References

• Biomarkers, Tumor
• MicroRNAs
• Prostate-Specific Antigen

Topics

• Adenocarcinoma (chemistry, diagnosis, urine)
• Aged
• Biomarkers, Tumor (analysis, urine)
• Follow-Up Studies
• Frozen Sections
• Gene Expression Profiling
• Humans
• Male
• MicroRNAs (analysis, urine)
• Middle Aged
• Neoplasm Grading
• Prospective Studies
• Prostate-Specific Antigen (blood)
• Prostatectomy
• Prostatic Hyperplasia (urine)
• Prostatic Neoplasms (chemistry, diagnosis, urine)
• ROC Curve
• Reverse Transcriptase Polymerase Chain Reaction
• Sensitivity and Specificity