Cervical cancer is a cause of
cancer death, making it as the one of the most common cause for death among women globally. Though many studies before have explored a lot for
cervical cancer prevention and treatment, there are still a lot far from to know based on the molecular mechanisms.
Janus kinase 2 (JAK2) has been reported to play an essential role in the progression of apoptosis, autophagy and proliferation for cells. We loaded
gold-
quercetin into
poly (dl-lactide-co-glycolide) nanoparticles to
cervical cancer cells due to the propertities of
quercetin in ameliorating cellular processes and the easier absorbance of nanoparticles. Here, in our study,
quercetin nanoparticles (NQ) were administrated to cells to investigate the underlying mechanism by which the
cervical cancer was regulated. First, JAK2-inhibited carvical
cancer cell lines were involved for our experiments in vitro and in vivo. Western blotting, quantitative RT-PCR (qRT-PCR), ELISA, Immunohistochemistry, and flow-cytometric analysis were used to determine the key signaling pathway regulated by JAK2 for
cervical cancer progression. And the role of
quercetin nanoparticles was determined during the process. Data here indicated that JAK2, indeed, expressed highly in
cancer cell lines compared to the normal cervical cells. And apoptosis and autophagy were found in JAK2-inhibited
cancer cells through activating
Caspase-3, and suppressing Cyclin-D1 and mTOR regulated by Signal Transducer and Activator of Transcription (STAT) 3/5 and phosphatidylinositide 3-
kinase/protein kinases (PI3K/AKT) signaling pathway. The
cervical cancer cells proliferation was inhibited. Further,
tumor size and weight were reduced by inhibition of JAK2 in vivo experiments. Notably, administration with
quercetin nanoparticles displayed similar role with JAK2 suppression, which could inhibit
cervical cancer cells proliferation, invasion and migration. In addition, autophogy and apoptosis were induced, promoting
cervical cancer cell death. To our knowledge, it was the first time to evaluate the role of
quercetin nanoparticles in improving
cervical cancer from apoptosis, autophagy and proliferation, which could be a potential target for future therapeutic approach clinically.