Abstract |
Under phosphate (Pi ) starvation, plants increase the secretion of purple acid phosphatases (PAPs) into the rhizosphere to scavenge organic phosphorus (P) for plant use. To date, only a few members of the PAP family have been characterized in crops. In this study, we identified a novel secreted PAP in rice, OsPAP10c, and investigated its role in the utilization of external organic P. OsPAP10c belongs to a monocotyledon-specific subclass of Ia group PAPs and is specifically expressed in the epidermis/exodermis cell layers of roots. Both the transcript and protein levels of OsPAP10c are strongly induced by Pi starvation. OsPAP10c overexpression increased acid phosphatase (APase) activity by more than 10-fold in the culture media and almost fivefold in both roots and leaves under Pi -sufficient and Pi -deficient conditions. This increase in APase activity further improved the plant utilization efficiency of external organic P. Moreover, several APase isoforms corresponding to OsPAP10c were identified using in-gel activity assays. Under field conditions with three different Pi supply levels, OsPAP10c-overexpressing plants had significantly higher tiller numbers and shorter plant heights. This study indicates that OsPAP10c encodes a novel secreted APase that plays an important role in the utilization of external organic P in rice.
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Authors | Linghong Lu, Wenmin Qiu, Wenwen Gao, Stephen D Tyerman, Huixia Shou, Chuang Wang |
Journal | Plant, cell & environment
(Plant Cell Environ)
Vol. 39
Issue 10
Pg. 2247-59
(10 2016)
ISSN: 1365-3040 [Electronic] United States |
PMID | 27411391
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2016 John Wiley & Sons Ltd. |
Chemical References |
- Plant Proteins
- RNA, Messenger
- Phosphorus
- Acid Phosphatase
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Topics |
- Acid Phosphatase
(genetics, metabolism, physiology)
- Crops, Agricultural
(enzymology, genetics, metabolism)
- Gene Expression Regulation, Plant
- Oryza
(enzymology, genetics, metabolism)
- Phosphorus
(metabolism, pharmacology)
- Phylogeny
- Plant Leaves
(enzymology, genetics, metabolism)
- Plant Proteins
(genetics, metabolism, physiology)
- Plant Roots
(enzymology, genetics, metabolism)
- RNA, Messenger
(metabolism)
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