Abstract | BACKGROUND: METHODS: Male Sprague-Dawley rats were randomly assigned into one of the four groups of rodent models: naive, CS alone, combined LCHS, or LCHS/CS. Plasma was used to evaluate levels of EPO, hepcidin, iron, and ferritin. RNA was isolated from bone marrow and lung tissue to evaluate expression of EPOr. Comparisons between models were performed by t tests followed by one-way analysis of variance. RESULTS: After 7 days, only LCHS/CS was associated with persistent anemia despite significant elevation of plasma EPO. Combined LCHS and LCHS/CS led to a persistent decrease in EPOr expression in bone marrow on Day 7. The LCHS/CS significantly decreased plasma hepcidin levels by 75% on Day 1 and 84% on Day 7 compared to LCHS alone. Hepcidin plasma levels are inversely proportional to EPO plasma levels (Pearson R = -0.362, p < 0.05). CONCLUSION: Tissue injury, hemorrhagic shock, and stress stimulate and maintain high levels of plasma EPO while hepcidin levels are decreased. In addition, bone marrow EPOr and plasma iron availability are significantly reduced following LCHS/CS. The combined deficit of reduced iron availability and reduced bone marrow EPOr expression may play a key role in the ineffective EPO response associated with persistent injury-associated anemia.
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Authors | Ines G Alamo, Kolenkode B Kannan, Michael A Smith, Philip A Efron, Alicia M Mohr |
Journal | The journal of trauma and acute care surgery
(J Trauma Acute Care Surg)
Vol. 81
Issue 4
Pg. 705-12
(10 2016)
ISSN: 2163-0763 [Electronic] United States |
PMID | 27398985
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
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Topics |
- Anemia
(etiology, physiopathology)
- Animals
- Bone Marrow
(metabolism)
- Disease Models, Animal
- Enzyme-Linked Immunosorbent Assay
- Erythropoietin
(metabolism)
- Hepcidins
(metabolism)
- Lung Injury
(complications, physiopathology)
- Male
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Restraint, Physical
- Shock, Hemorrhagic
(complications, physiopathology)
- Stress, Physiological
(physiology)
- Wounds and Injuries
(blood, complications)
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