Abstract | BACKGROUND: MATERIALS AND METHODS: Eleven CLAWN miniature swine received MHC matched but minor antigen mismatched allogenic intestinal grafts. Four animals received intestinal grafts heterotopically and kept host intestine intact. The remaining 7 animals received intestinal grafts orthotopically and resected host small intestine. Continuous infusion of tacrolimus was given from day 0 for 12 days. RESULTS: Heterotopically transplanted small intestine were well perfused after revascularization; however, grafts easily underwent ischemic changes during or soon after abdomen closure due to oppression of the grafts in the limited abdominal space. In contrast, all of 7 orthotopically transplanted intestinal grafts in which recipients' small intestine was removed from the jejunum to the ileum had no signs of severe ischemia associated with compartment syndrome. Elevation of the serum concentration of inflammatory cytokines and the progression of lethal acidosis seen in recipients of heterotipic transplantation were markedly less in the case of orthotopic transplantation. Two recipients survived more than 30 days, and 1 long-term survivor showed no evidence of rejection at day 90 despite the fact that tacrolimus was stopped at day 12. CONCLUSIONS: In this study, we demonstrated the establishment of a clinically relevant orthotopic Int-Tx model with long survival in MHC inbred CLAWN miniature swine. We believe that this unique MHC inbred swine Int-Tx model is useful for developing treatment strategies for clinical Int-Tx.
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Authors | K Miura, H Sahara, S Waki, A Kawai, M Sekijima, T Kobayashi, Z Zhang, T Wakai, A Shimizu, K Yamada |
Journal | Transplantation proceedings
(Transplant Proc)
Vol. 48
Issue 4
Pg. 1315-9
(May 2016)
ISSN: 1873-2623 [Electronic] United States |
PMID | 27320612
(Publication Type: Journal Article)
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Copyright | Copyright © 2016 Elsevier Inc. All rights reserved. |
Chemical References |
- Immunosuppressive Agents
- Tacrolimus
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Topics |
- Animals
- Disease Models, Animal
- Graft Rejection
(prevention & control)
- Graft vs Host Disease
(prevention & control)
- Ileum
(surgery)
- Immunosuppressive Agents
(administration & dosage, pharmacology)
- Infusions, Intravenous
- Intestine, Small
(transplantation)
- Jejunum
(surgery)
- Major Histocompatibility Complex
(physiology)
- Reperfusion Injury
(prevention & control)
- Swine
- Swine, Miniature
- Tacrolimus
(administration & dosage)
- Transplantation, Heterotopic
(methods)
- Transplantation, Homologous
(methods)
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