In Vivo Magnetic Resonance and Fluorescence Dual-Modality Imaging of Tumor Angiogenesis in Rats Using GEBP11 Peptide Targeted Magnetic Nanoparticles.

Abstract

Angiogenesis is an essential process for tumor progression. Tumor vasculature-targeting peptides have shown great potential for use in cancer imaging and therapy. Our previous studies have shown that GEBP11, a novel vasculature-specific binding peptide that exhibits high affinity and specificity to tumor angiogenesis, is a promising candidate for the diagnosis and targeted radiotherapy of gastric cancer. In the present study, we developed a novel magnetic resonance and fluorescence (MR/Fluo) dual-modality imaging probe by covalently coupling 2,3-dimercaptosuccinnic acid-coated paramagnetic nanoparticles (DMSA-MNPs) and Cy5.5 to the GEBP11 peptide. The probe Cy5.5-GEBP11-DMSA-MNPs (CGD-MNPs), with a hydrodynamic diameter of 82.8 ± 6.5 nm, exhibited good imaging properties, high stability and little cytotoxicity. In vivo MR/Fluo imaging revealed that CGD-MNPs were successfully applied to visualize tumor angiogenesis in SGC-7901 xenograft mouse models. Prussian blue and CD31 immunohistochemical staining confirmed that CGD-MNPs co-localized with tumor blood vessels. In conclusion, CGD-MNPs are promising candidates for use as MR and fluorescence imaging probes for visualizing gastric cancer angiogenesis in vivo.

• Authors: Tao Su, Yabin Wang, Jiinda Wang, Dong Han, Sai Ma, Jianbo Cao, Xiujuan Li, Ran Zhang, Hongyu Qiao, Jimin Liang, Gang Liu, Bo Yang, Shuhui Liang, Yongzhan Nie, Kaichun Wu, Jiayi Li, Feng Cao
• Journal: Journal of biomedical nanotechnology (J Biomed Nanotechnol) Vol. 12 Issue 5 Pg. 1011-22 (May 2016) ISSN: 1550-7033 [Print] United States
• PMID: 27305822 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CY5.5 cyanine dye
• Carbocyanines
• GEBP11 peptide
• Magnetite Nanoparticles
• Peptides
• Succimer

Topics

• Animals
• Carbocyanines (metabolism)
• Cell Death (drug effects)
• Diagnostic Imaging
• Endocytosis (drug effects)
• Fluorescence
• Human Umbilical Vein Endothelial Cells (drug effects, metabolism)
• Humans
• Magnetic Resonance Imaging
• Magnetic Resonance Spectroscopy
• Magnetite Nanoparticles (chemistry, toxicity, ultrastructure)
• Mice, Nude
• Neoplasms (blood supply, pathology)
• Neovascularization, Pathologic (diagnosis)
• Peptides (pharmacology)
• Rats
• Succimer (metabolism)
• Tissue Distribution (drug effects)