MicroRNA-200b Impacts Breast Cancer Cell Migration and Invasion by Regulating Ezrin-Radixin-Moesin.

Abstract

BACKGROUND Ezrin-radixin-moesin (ERM) plays an important role in multiple links of tumors. It also involved in breast cancer invasion and metastasis, and might be a potential biomarker of breast cancer. Another study suggested that ERM expression was regulated directly by miR-200c, and had a critical role in miR-200c suppressing cell migration. This study aimed to investigate the effect of miR-200b on ERM expression in a breast cancer cell line and its influence on invasion and metastasis ability in vitro. MATERIAL AND METHODS Breast cancer cell lines MCF-7 and MDA-MB-231 with different metastatic potentials were selected as a model. MiR-200b overexpression or inhibition was achieved by Lipofectamine™ 2000-mediated miRNA transfection. RT-PCR was used to test miR-200b level, while Western blot was selected to detect ERM protein expression. Wound healing assay and Transwell assay were performed to determine cell migration and invasion ability. RESULTS RT-PCR revealed that miR-200b level in MDA-MB-231 was obviously lower than that in MCF-7, while Western blot analysis showed that ERM expression was significantly higher. MiR-200b inhibition by transfection in MCF-7 markedly decreased miR-200b level, elevated ERM expression, and enhanced cell migration and invasion. MiR-200b overexpression in MDA-MB-231 obviously increased miR-200b level, reduced ERM expression, and weakened cell migration and invasion. CONCLUSIONS MiR-200b participates in breast cancer cell migration and invasion through regulating ERM in MCF-7 and MDA-MB-231.

• Authors: Hong Hong, Haizhong Yu, Jianfen Yuan, Chunyan Guo, Hongyan Cao, Weibing Li, Chunhong Xiao
• Journal: Medical science monitor : international medical journal of experimental and clinical research (Med Sci Monit) Vol. 22 Pg. 1946-52 (Jun 08 2016) ISSN: 1643-3750 [Electronic] United States
• PMID: 27276064 (Publication Type: Journal Article)

Chemical References

• DNA-Binding Proteins
• ETV5 protein, human
• MIRN200 microRNA, human
• MicroRNAs
• Transcription Factors
• Amlodipine

Topics

• Amlodipine (metabolism)
• Breast Neoplasms (genetics, metabolism, pathology)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• DNA-Binding Proteins (biosynthesis, genetics, metabolism)
• Female
• Humans
• MCF-7 Cells
• MicroRNAs (administration & dosage, biosynthesis, genetics, metabolism)
• Neoplasm Invasiveness
• Transcription Factors (biosynthesis, genetics, metabolism)
• Transfection