Abstract |
The nutritional compound capsaicin inhibits the invasion of many types of human cancers. The clinical development of capsaicin as an anti- cancer drug is limited due to its unfavorable side effects like burning sensation, stomach cramps, gut pain and nausea. This study compared the anti-invasive activity of capsaicin to non-pungent long chain capsaicin analogs, namely arvanil and olvanil, in human small cell lung cancer cells. Boyden chamber invasion assays revealed that arvanil and olvanil displayed improved anti-invasive activity relative to capsaicin in human SCLC cells. The results of the Boyden chamber assay were confirmed by the spherical invasion assay, and similar results were obtained. The anti-invasive activity of arvanil, olvanil and capsaicin were independent of TRPV and CB1 receptors. Furthermore, the anti-invasive activity of arvanil, olvanil and capsaicin was mediated by the AMPK pathway. Depletion of AMPK levels by siRNA methodology abrogated the anti-invasive activity of arvanil, olvanil and capsaicin. The non-pungent capsaicin analogs arvanil and olvanil display improved anti-invasive activity relative to capsaicin in human SCLC cells. These agents may represent the second generation of capsaicin-like compounds which are more potent than the parent molecule and have a better side effect profile.
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Authors | John D Hurley, Austin T Akers, Jamie R Friedman, Nicholas A Nolan, Kathleen C Brown, Piyali Dasgupta |
Journal | Cell adhesion & migration
(Cell Adh Migr)
Vol. 11
Issue 1
Pg. 80-97
(01 02 2017)
ISSN: 1933-6926 [Electronic] United States |
PMID | 27196129
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural)
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Chemical References |
- Receptor, Cannabinoid, CB1
- TRPV Cation Channels
- arvanil
- olvanil
- Adenylate Kinase
- Capsaicin
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Topics |
- Adenylate Kinase
(metabolism)
- Animals
- Capsaicin
(analogs & derivatives, chemistry, pharmacology)
- Cell Line, Tumor
- Humans
- Lung Neoplasms
(pathology)
- Neoplasm Invasiveness
- Receptor, Cannabinoid, CB1
(metabolism)
- Signal Transduction
(drug effects)
- Small Cell Lung Carcinoma
(pathology)
- TRPV Cation Channels
(metabolism)
- Time Factors
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