Abstract | OBJECTIVE: DESIGN: A primary, experimental, analytical, prospective, self-controlled, blinded study. SETTING: The study is set in a university research center. INTERVENTIONS: Twenty-eight Wistar rats were randomly divided into 4 groups: lidocaine, bupivacaine, ropivacaine, and the control. After general anesthesia, a local anesthetic or 0.9% saline (control) was injected subdermally along a 2-cm line on the dorsal midline of each rat; 30 minutes later, an incision (nociceptive stimulus) was made along this line. The animals were euthanized, and skin samples were collected from the center of the incision line and sent for CGRP and SP quantification. MAIN OUTCOME MEASURES: Quantification of CGRP and SP by Western blotting. RESULTS:
Substance P levels were similar in the lidocaine and ropivacaine groups but were significantly lower than those of the control group (P = .002); no significant difference in SP levels was found between the bupivacaine and control groups. Procalcitonin gene-related peptide levels were significantly lower in the experimental groups than those in control subjects (P = .009), with no significant differences among the experimental groups. No significant differences in CGRP levels were found among all groups. Lidocaine and ropivacaine inhibited SP release. All 3 local anesthetics inhibited the release of procalcitonin gene-related peptide, but not the release of CGRP in rat skin. CONCLUSIONS:
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Authors | Guilherme A F Lapin, Bernardo Hochman, Jessica R Maximino, Gerson Chadi, Lydia M Ferreira |
Journal | Advances in skin & wound care
(Adv Skin Wound Care)
Vol. 29
Issue 4
Pg. 169-77
(Apr 2016)
ISSN: 1538-8654 [Electronic] United States |
PMID | 26978801
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Amides
- Anesthetics, Local
- Substance P
- Ropivacaine
- Lidocaine
- Calcitonin Gene-Related Peptide
- Bupivacaine
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Topics |
- Amides
(pharmacology)
- Anesthetics, Local
(pharmacology)
- Animals
- Bupivacaine
(pharmacology)
- Calcitonin Gene-Related Peptide
(metabolism)
- Lidocaine
(pharmacology)
- Rats
- Rats, Wistar
- Ropivacaine
- Skin
(drug effects, injuries, metabolism)
- Substance P
(metabolism)
- Surgical Wound
(metabolism)
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