Abstract | OBJECTIVE: Intravenous or intraspinal transplantation of human umbilical cord blood cells-derived CD34(+) cells (human CD34(+) cells) or mesenchymal stem cells after spinal cord injury (SCI) improved hind limb functional recovery in adult rats. The objective of this study is to ascertain whether SCI in rats can be attenuated by conditioned medium (CM) or secretome obtained from cultured human CD34(+) stem cells. MATERIALS AND METHODS: Sprague-Dawley rats were assigned to one of the following five groups: the sham group, the SCI group treated with vehicle solution (SCI + V), the SCI group treated with CM (SCI + CM), the SCI group treated with 17β-estradiol E2 (10 μg; SCI + E2), and the SCI group treated with CM plus E2 (SCI + CM + E2). A 0.5-mL volume of CM or vehicle solution was administered intravenously immediately after SCI. RESULTS: Compared with the sham group, the (SCI + V) group had significantly higher scores of neurological motor dysfunction as well as inflammation apoptosis, oxidative stress, and astrogliosis in the injured spinal cord. The neurological deficits, numbers of apoptotic cell, extent of inflammation, oxidative stress, and astrogliosis in the injured spinal cord were significantly attenuated by CM, E2, or CM plus E2, but not by the vehicle solution. In addition, the neuroprotective effect exerted by a combination of CM and E2 is superior to that exerted by CM- or E2-alone therapy. CONCLUSION:
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Authors | Chia-Hong Yeng, Pei-Jarn Chen, Hsiu-Kang Chang, Wei-Yu Lo, Chia-Chun Wu, Chia-Yu Chang, Chih-Ho Chou, Sheng-Hsien Chen |
Journal | Taiwanese journal of obstetrics & gynecology
(Taiwan J Obstet Gynecol)
Vol. 55
Issue 1
Pg. 85-93
(Feb 2016)
ISSN: 1875-6263 [Electronic] China (Republic : 1949- ) |
PMID | 26927256
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2016. Published by Elsevier B.V. |
Chemical References |
- Antigens, CD34
- Culture Media, Conditioned
- Estrogens
- Estradiol
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Topics |
- Animals
- Antigens, CD34
(analysis)
- Apoptosis
(drug effects)
- Cells, Cultured
- Culture Media, Conditioned
(pharmacology)
- Drug Synergism
- Drug Therapy, Combination
- Estradiol
(pharmacology, therapeutic use)
- Estrogens
(pharmacology, therapeutic use)
- Fetal Blood
(cytology)
- Fetal Stem Cells
(chemistry, metabolism)
- Humans
- Inflammation
(drug therapy)
- Locomotion
(drug effects)
- Male
- Oxidative Stress
(drug effects)
- Rats
- Rats, Sprague-Dawley
- Spinal Cord Injuries
(complications, drug therapy, physiopathology)
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