Influence of Interleukin-1 Beta on Platelet-Poor Plasma Clot Formation: A Potential Impact on Early Bone Healing.

Abstract

OBJECTIVES:

Hematoma quality (especially the fibrin matrix) plays an important role in the bone healing process. Here, we investigated the effect of interleukin-1 beta (IL-1β) on fibrin clot formation from platelet-poor plasma (PPP).

METHODS:

Five-milliliter of rat whole-blood samples were collected from the hepatic portal vein. All blood samples were firstly standardized via a thrombelastograph (TEG), blood cell count, and the measurement of fibrinogen concentration. PPP was prepared by collecting the top two-fifths of the plasma after centrifugation under 400 × g for 10 min at 20°C. The effects of IL-1β cytokines on artificial fibrin clot formation from PPP solutions were determined by scanning electronic microscopy (SEM), confocal microscopy (CM), turbidity, and clot lysis assays.

RESULTS:

The lag time for protofibril formation was markedly shortened in the IL-1β treatment groups (243.8 ± 76.85 in the 50 pg/mL of IL-1β and 97.5 ± 19.36 in the 500 pg/mL of IL-1β) compared to the control group without IL-1β (543.8 ± 205.8). Maximal turbidity was observed in the control group. IL-1β (500 pg/mL) treatment significantly decreased fiber diameters resulting in smaller pore sizes and increased density of the fibrin clot structure formed from PPP (P < 0.05). The clot lysis assay revealed that 500 pg/mL IL-1β induced a lower susceptibility to dissolution due to the formation of thinner and denser fibers.

CONCLUSION:

IL-1β can significantly influence PPP fibrin clot structure, which may affect the early bone healing process.

• Authors: Xin Wang, Yan Luo, Paul P Masci, Ross Crawford, Yin Xiao
• Journal: PloS one (PLoS One) Vol. 11 Issue 2 Pg. e0149775 ( 2016) ISSN: 1932-6203 [Electronic] United States
• PMID: 26909757 (Publication Type: Journal Article)

Chemical References

• IL1B protein, rat
• Interleukin-1beta

Topics

• Animals
• Blood Coagulation
• Fracture Healing
• Hematoma (metabolism, pathology)
• Humans
• Interleukin-1beta (metabolism)
• Rats
• Rats, Inbred F344