Molecular Characterization of a Prevalent Ribocluster of Methicillin-Sensitive Staphylococcus aureus from Orthopedic Implant Infections. Correspondence with MLST CC30.

Abstract

Staphylococcus aureus is the leading etiologic agent of orthopedic implant infections. Here a ribocluster of 27 S. aureus strains underwent further molecular characterization and subtyping by multilocus sequence typing (MLST) and spa-typing. This cluster had been detected by automated ribotyping (with the EcoRI restriction enzyme) of 200 S. aureus isolates from periprosthetic infections of patients who underwent revision at the Rizzoli Orthopaedic Institute. The ribocluster, consisting of agr type III strains, with a 74% co-occurrence of bone sialoprotein-binding (bbp) and collagen-binding (cna) genes, lacked mecA and IS256, and exhibited a high prevalence of the toxic shock syndrome toxin gene (tst, 85%). Strains' relatedness was analyzed by BURP and eBURST. Two predominant spa types, t012 (32%) and t021 (36%), and one predominant sequence type, ST30 (18/27, 67%) were identified: a S. aureus lineage spread worldwide belonging to MLST CC30. Two new sequence types (ST2954, ST2960) and one new spa type (t13129) were detected for the first time. Interestingly, the 27-strain cluster detected by ribotyping corresponded exactly to MLST CC30, the sole CC identified by eBURST.

• Authors: Lucio Montanaro, Stefano Ravaioli, Werner Ruppitsch, Davide Campoccia, Giampiero Pietrocola, Livia Visai, Pietro Speziale, Franz Allerberger, Carla Renata Arciola
• Journal: Frontiers in cellular and infection microbiology (Front Cell Infect Microbiol) Vol. 6 Pg. 8 ( 2016) ISSN: 2235-2988 [Electronic] Switzerland
• PMID: 26909340 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adhesins, Bacterial
• Anti-Bacterial Agents
• Bacterial Proteins
• Bacterial Toxins
• Carrier Proteins
• Enterotoxins
• Penicillin-Binding Proteins
• Superantigens
• Virulence Factors
• adhesin, Staphylococcus aureus
• bone sialoprotein-binding protein, Staphylococcus aureus
• enterotoxin F, Staphylococcal
• mecA protein, Staphylococcus aureus

Topics

• Adhesins, Bacterial (genetics)
• Anti-Bacterial Agents (pharmacology)
• Bacterial Proteins (genetics)
• Bacterial Toxins (genetics)
• Carrier Proteins (genetics)
• Drug Resistance, Bacterial (genetics)
• Enterotoxins (genetics)
• Humans
• Microbial Sensitivity Tests
• Multilocus Sequence Typing
• Penicillin-Binding Proteins (genetics)
• Prosthesis-Related Infections (microbiology)
• Ribotyping
• Staphylococcal Infections (microbiology)
• Staphylococcus aureus (drug effects, genetics, isolation & purification, pathogenicity)
• Superantigens (genetics)
• Virulence Factors (genetics)