Surface plasmon resonance immunosensor for ErbB2 breast cancer biomarker determination in human serum and raw cancer cell lysates.

Abstract

A highly sensitive surface plasmon resonance (SPR) immunosensor for the important ErbB2 breast cancer biomarker has been developed. Optimization of the assay has been carried out, including signal enhancement employing gold nanoparticles (GNPs). The effect of the signal amplification of the GNPs has been also studied. The assay has been tested with clinically relevant matrices. Results in 50% human serum yielded a LOD of 180 pg mL(-1) which is a concentration 83 times lower than the clinical cut-off. Raw lysates from model breast cancer cell lines (SK-BR-3, MCF-7 and MDA-MB-436) have been also assayed and higher quantities of the ErbB2 protein were clearly observed in the ErbB2 over-expressing case (SK-BR-3). The results confirmed that the simple and highly sensitive SPR immunosensor represents a feasible tool for ErbB2 detection.

• Authors: Unai Eletxigerra, Josu Martinez-Perdiguero, Rodrigo Barderas, José M Pingarrón, Susana Campuzano, Santos Merino
• Journal: Analytica chimica acta (Anal Chim Acta) Vol. 905 Pg. 156-62 (Jan 28 2016) ISSN: 1873-4324 [Electronic] Netherlands
• PMID: 26755150 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.

Chemical References

• Biomarkers, Tumor
• ERBB2 protein, human
• Receptor, ErbB-2

Topics

• Biomarkers, Tumor (blood, metabolism)
• Biosensing Techniques
• Breast Neoplasms (diagnosis, pathology)
• Cell Line, Tumor
• Female
• Humans
• Receptor, ErbB-2 (blood, metabolism)
• Surface Plasmon Resonance (methods)