The role of TRPV1 in the CD4+ T cell-mediated inflammatory response of allergic rhinitis.

Abstract

Transient receptor potential vanilloid 1 (TRPV1), which has been identified as a molecular target for the activation of sensory neurons by various painful stimuli, was reported to regulate the signaling and activation of CD4+ T cells. However, the role of TRPV1 in CD4+ T cell in allergic rhinitis remains poorly understood. In this study, TRPV1 expression was localized in CD4+ T cells. Both knockout and chemical inhibition of TRPV1 suppressed Th2/Th17 cytokine production in CD4 T cells and Jurkat T cells, respectively, and can suppress T cell receptor signaling pathways including NF-κB, MAP kinase, and NFAT. In TRPV1 knockout allergic rhinitis (AR) mice, eosinophil infiltration, Th2/Th17 cytokines in the nasal mucosa, and total and ova-specific IgE levels in serum decreased, compared with wild-type AR mice. The TRPV1 antagonists, BCTC or theobromine, showed similar inhibitory immunologic effects on AR mice models. In addition, the number of TRPV1+/CD4+ inflammatory cells increased in the nasal mucosa of patients with AR, compared with that of control subjects. Thus, TRPV1 activation on CD4+ T cells is involved in T cell receptor signaling, and it could be a novel therapeutic target in AR.

• Authors: Ramachandran Samivel, Dae Woo Kim, Hye Ran Son, Yun-Hee Rhee, Eun Hee Kim, Ji Hye Kim, Jun-Sang Bae, Young-Jun Chung, Phil-Sang Chung, Eyal Raz, Ji-Hun Mo
• Journal: Oncotarget (Oncotarget) Vol. 7 Issue 1 Pg. 148-60 (Jan 05 2016) ISSN: 1949-2553 [Electronic] United States
• PMID: 26700618 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• TRPV Cation Channels
• Immunoglobulin E
• Ovalbumin

Topics

• Adolescent
• Adult
• Animals
• Blotting, Western
• CD4-Positive T-Lymphocytes (immunology, metabolism)
• Cytokines (genetics, immunology, metabolism)
• Eosinophils (immunology, metabolism)
• Female
• Humans
• Immunoglobulin E (blood, immunology)
• Immunohistochemistry
• Inflammation (genetics, immunology, metabolism)
• Jurkat Cells
• Mice, Inbred BALB C
• Mice, Inbred C57BL
• Mice, Knockout
• Middle Aged
• Nasal Mucosa (immunology, metabolism)
• Ovalbumin (immunology)
• Reverse Transcriptase Polymerase Chain Reaction
• Rhinitis, Allergic (genetics, immunology, metabolism)
• TRPV Cation Channels (genetics, immunology, metabolism)
• Young Adult