Abstract | BACKGROUND: METHODS: We used immunohistochemistry (IHC) and reverse transcriptase-polymerase chain reaction (RT-PCR) to evaluate the expressions of CXCL12, CXCR4, and CXCR7 in ESCC patients' tumor biopsy specimens obtained during preoperative endoscopy or surgery. These results were compared with the patients' clinicopathological parameters and survival. RESULTS: IHC was conducted for 172 patients. High expression of CXCR4 in the cytoplasm and nuclei and that of CXCR7 were associated with poor cause-specific survival (CSS) (P= .002 and .010, respectively). The specimens from 52 of the 172 patients were examined by RT-PCR and quantitative real-time PCR. The expression levels of messenger RNA ( mRNA) of CXCR4 and CXCR7 were significantly increased in the tumors compared with normal esophageal mucosae (P < .0001). The expression level of mRNA of CXCR4 was associated with poor recurrence-free survival and CSS (P = .012 and .038, respectively). CONCLUSIONS: CXCR4 expression is associated with poor prognosis in patients with ESCC.
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Authors | Masakazu Goto, Takahiro Yoshida, Yota Yamamoto, Yoshihito Furukita, Seiya Inoue, Satoshi Fujiwara, Naoya Kawakita, Takeshi Nishino, Takuya Minato, Yasuhiro Yuasa, Hiromichi Yamai, Hirokazu Takechi, Junichi Seike, Yoshimi Bando, Akira Tangoku |
Journal | Annals of surgical oncology
(Ann Surg Oncol)
Vol. 24
Issue 3
Pg. 832-840
(Mar 2017)
ISSN: 1534-4681 [Electronic] United States |
PMID | 26577115
(Publication Type: Journal Article)
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Chemical References |
- ACKR3 protein, human
- CXCL12 protein, human
- CXCR4 protein, human
- Chemokine CXCL12
- RNA, Messenger
- Receptors, CXCR
- Receptors, CXCR4
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Topics |
- Antineoplastic Combined Chemotherapy Protocols
(therapeutic use)
- Carcinoma, Squamous Cell
(genetics, metabolism, therapy)
- Chemokine CXCL12
(genetics, metabolism)
- Disease-Free Survival
- Esophageal Neoplasms
(genetics, metabolism, therapy)
- Esophagectomy
- Female
- Gene Expression
- Humans
- Male
- Middle Aged
- RNA, Messenger
(metabolism)
- Receptors, CXCR
(genetics, metabolism)
- Receptors, CXCR4
(genetics, metabolism)
- Retrospective Studies
- Survival Rate
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