Anti-inflammatory effects of vicenin-2 and scolymoside in vitro and in vivo.

Abstract

AIM AND OBJECTIVE:

Two structurally related flavonoids found in Cyclopia subternata, namely vicenin-2 and scolymoside, were examined for its effects on inflammatory responses by monitoring the effects of vicenin-2 and scolymoside on lipopolysaccharide (LPS)-mediated vascular inflammatory responses.

METHODS:

The anti-inflammatory activities of vicenin-2 and scolymoside were determined by measuring permeability,monocytes adhesion and migration, and activation of pro-inflammatory proteins in LPS-activated HUVECs and mice.

RESULTS:

We found that post-treatment of each compound inhibited LPS-induced barrier disruption, expression of cell adhesion molecules (CAMs), and adhesion/transendothelial migration of human neutrophils to human endothelial cells. Each compound induced potent inhibition of phorbol-12-myristate 13-acetate (PMA) and LPS-induced endothelial cell protein C receptor (EPCR)shedding. It also suppressed LPS-induced hyperpermeability and leukocytes migration in vivo. Furthermore,each compound suppressed the production of tumor necrosis factor-α (TNF-α) or Interleukin (IL)-6 and the activation of nuclear factor-κB (NF-κB) or extracellular regulated kinases (ERK) 1/2 by LPS. Moreover, posttreatment with each compound resulted in reduced LPS-induced lethal endotoxemia.

CONCLUSION:

Vicenin-2 and scolymoside possess anti-inflammatory functions by inhibiting hyperpermeability,expression of CAMs, and adhesion and migration of leukocytes, thereby endorsing its usefulness as a therapy for vascular inflammatory diseases.

• Authors: Hyejin Kang, Sae-Kwang Ku, Byeongjin Jung, Jong-Sup Bae
• Journal: Inflammation research : official journal of the European Histamine Research Society ... [et al.] (Inflamm Res) Vol. 64 Issue 12 Pg. 1005-21 (Dec 2015) ISSN: 1420-908X [Electronic] Switzerland
• PMID: 26482935 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Glucosides
• Interleukin-6
• Lipopolysaccharides
• apigenin-6,8-di-C-glycopyranoside
• interleukin-6, mouse
• scolymoside
• Apigenin
• ADAM Proteins
• ADAM17 Protein
• Luteolin
• Tetradecanoylphorbol Acetate

Topics

• ADAM Proteins (biosynthesis, genetics)
• ADAM17 Protein
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Apigenin (pharmacology)
• Cell Adhesion (drug effects)
• Cell Membrane Permeability (drug effects)
• Cell Movement (drug effects)
• Glucosides (pharmacology)
• Human Umbilical Vein Endothelial Cells (drug effects)
• Humans
• Interleukin-6 (biosynthesis)
• Lipopolysaccharides (antagonists & inhibitors, pharmacology)
• Luteolin (pharmacology)
• Macrophage Activation (drug effects)
• Male
• Mice
• Mice, Inbred C57BL
• Monocytes (drug effects)
• Tetradecanoylphorbol Acetate (antagonists & inhibitors)