Astragaloside IV attenuates apoptosis of hypertrophic cardiomyocyte through inhibiting oxidative stress and calpain-1 activation.

Abstract

Calpain-1 activation and oxidative stress are two critical factors contributing to apoptosis of hypertrophic cardiomyocyte. Astragaloside IV (ASIV) exhibits protective effect against various heart diseases. The present study was designed to investigate whether the inhibitory effect of ASIV on isoproterenol (ISO)-induced apoptosis of hypertrophic cardiomyocyte was associated with the anti-oxidation and calpain-1 inhibition. Hypertrophy, apoptosis, mitochondrial oxidative stress and calpain-1 expression were measured in the heart tissue of Sprague-Dawley (SD) rats and H9C2 cells treated with ISO alone or combination with ASIV. The results showed that ASIV attenuated apoptotic rate, increased Bcl-2 expression, decreased Bax expression, ameliorated the integrity of mitochondrial structure and improved mitochondrial membrane potential (MMP). Moreover, ASIV combination reduced both calpain-1 protein expression and calpain activity, down-regulated mitochondrial NOX4 (mito-NOX4) expression, increased activity of mitochondrial superoxide dismutase (mito-SOD) and mitochondrial catalase (mito-CAT) compared to ISO treated alone. The results suggested that ASIV exerted anti-apoptosis effect on ISO-induced hypertrophic cardiomyocyte by attenuating oxidative stress and calpain-1 activation.

• Authors: Meng Mei, Futian Tang, Meili Lu, Xin He, Hongxin Wang, Xuwei Hou, Jin Hu, Chonghua Xu, Ronghui Han
• Journal: Environmental toxicology and pharmacology (Environ Toxicol Pharmacol) Vol. 40 Issue 3 Pg. 764-73 (Nov 2015) ISSN: 1872-7077 [Electronic] Netherlands
• PMID: 26433482 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.

Chemical References

• Antioxidants
• Cardiotonic Agents
• Saponins
• Triterpenes
• astragaloside A
• Calpain
• Capn1 protein, rat
• 2-Propanol

Topics

• 2-Propanol (toxicity)
• Animals
• Antioxidants (administration & dosage, pharmacology)
• Apoptosis (drug effects)
• Calpain (metabolism)
• Cardiomegaly (chemically induced, metabolism, prevention & control)
• Cardiotonic Agents (pharmacology)
• Cell Line
• Gene Expression Regulation (drug effects)
• Membrane Potential, Mitochondrial (drug effects)
• Mitochondria (drug effects)
• Myocytes, Cardiac (cytology, drug effects, pathology)
• Oxidative Stress (drug effects)
• Rats
• Rats, Sprague-Dawley
• Saponins (administration & dosage, pharmacology)
• Triterpenes (administration & dosage, pharmacology)