Abstract | OBJECTIVE: METHODS: Overexpression or knockdown of ANGPTL4 was performed in WM-115 or WM-266-4 cells, respectively. The expression of ANGPTL4 and ALDOA was measured by RT-PCR and Western blot, respectively. The promoter activity of ALDOA gene was determined by luciferase assay.
RESULTS: The promoter activity of ALDOA gene and the expression of ALDOA ( mRNA and protein) were increased or decreased in the melanoma cells with overexpression or knockdown of ANGPTL4, which was blocked by selective protein kinase C (PKC) inhibitor or restored by PKC agonist, respectively.
CONCLUSION: ANGPTL4 promotes ALDOA expression in human melanoma cell in a PKC dependent manner.
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Authors | Yang Sun, Xiancheng Wang, Borong Fang, Xiang Xiong, Jianhong Long |
Journal | Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
(Zhong Nan Da Xue Xue Bao Yi Xue Ban)
Vol. 40
Issue 8
Pg. 851-7
(Aug 2015)
ISSN: 1672-7347 [Print] China |
PMID | 26333492
(Publication Type: Journal Article)
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Chemical References |
- ANGPTL4 protein, human
- Angiopoietin-Like Protein 4
- Angiopoietins
- RNA, Messenger
- Fructose-Bisphosphate Aldolase
|
Topics |
- Angiopoietin-Like Protein 4
- Angiopoietins
(genetics, metabolism)
- Blotting, Western
- Cell Line, Tumor
- Fructose-Bisphosphate Aldolase
(metabolism)
- Gene Knockdown Techniques
- Humans
- Melanoma
(genetics, metabolism)
- RNA, Messenger
(genetics, metabolism)
|