AGXT2L1 is down-regulated in heptocellular carcinoma and associated with abnormal lipogenesis.

Abstract

AIMS:

To clarify the clinical implications and functional role of the alanine-glyoxylate aminotransferase 2-like 1 (AGXT2L1) gene in hepatocellular carcinoma (HCC).

METHODS AND RESULTS:

We confirmed that AGXT2L1 was down-regulated in liver cancer samples by immunohistochemical (IHC) staining. We also demonstrated that this down-regulation was associated with several clinicopathological features such as alpha fetoprotein (AFP) serum level and T stage. Furthermore, we showed with Kaplan-Meier analysis that expression of AGXT2L1 in tumour samples was significantly correlated with patient prognosis. The bioinformatic tool indicated that AGXT2L1 plays a role in the lipid metabolic process of HCC tissue, while siRNA silenced the expression of AGXT2L1 in HCC 97H and LM3 cells, confirming that down-regulation of AGXT2L1 promotes the lipogenesis of cancer cells.

CONCLUSIONS:

For the first time, we have shown that AGXT2L1 is down-regulated in HCC and its low expression indicates a poor prognosis. Our findings also demonstrated that AGXT2L1 is a crucial gene in the abnormal lipogenesis of HCC tissue.

• Authors: Qianshan Ding, Jian Kang, Jinfen Dai, Meng Tang, Qi Wang, Haotian Zhang, Wenyi Guo, Rongze Sun, Honggang Yu
• Journal: Journal of clinical pathology (J Clin Pathol) Vol. 69 Issue 3 Pg. 215-20 (Mar 2016) ISSN: 1472-4146 [Electronic] England
• PMID: 26294768 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

Chemical References

• AFP protein, human
• alpha-Fetoproteins
• Transaminases
• Alanine-glyoxylate transaminase

Topics

• Carcinoma, Hepatocellular (enzymology, genetics, mortality, pathology, therapy)
• Cell Line, Tumor
• Computational Biology
• Databases, Genetic
• Down-Regulation
• Female
• Gene Expression Regulation, Enzymologic
• Gene Expression Regulation, Neoplastic
• Humans
• Kaplan-Meier Estimate
• Lipogenesis
• Liver Neoplasms (enzymology, genetics, mortality, pathology, therapy)
• Male
• Middle Aged
• Neoplasm Staging
• RNA Interference
• Signal Transduction
• Time Factors
• Transaminases (genetics, metabolism)
• Transfection
• Treatment Outcome
• alpha-Fetoproteins (analysis)